Proteolytic Cleavage of the p65-RelA Subunit of NF-κB during Poliovirus Infection
| Autor: | Amiya K. Banerjee, Lubov Neznanova, Andrei V. Gudkov, Nickolay Neznanov, Alexandru Almasan, Konstantin Chumakov |
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| Rok vydání: | 2005 |
| Předmět: |
viruses
Protein subunit Molecular Sequence Data Electrophoretic Mobility Shift Assay Biology medicine.disease_cause Cleavage (embryo) Biochemistry chemistry.chemical_compound medicine Protein biosynthesis Humans Amino Acid Sequence Molecular Biology Peptide sequence DNA Primers Innate immune system Base Sequence Hydrolysis Poliovirus NF-kappa B Transcription Factor RelA RNA NF-κB Cell Biology Virology Cell biology chemistry HeLa Cells Poliomyelitis |
| Zdroj: | Journal of Biological Chemistry. 280:24153-24158 |
| ISSN: | 0021-9258 |
| Popis: | Activation of NF-kappaB during viral infection is one of the critical elements in innate immune response. Several virus-specific factors, such as double-stranded RNA, can trigger host defense mechanisms by inducing NF-kappaB-mediated expression of cytokines and interferons. Early stages of poliovirus infection are also associated with degradation of IkappaB alpha and translocation of NF-kappaB into the nucleus. However, at later stages of poliovirus replication the p65-RelA component of the NF-kappaB complex undergoes a specific cleavage that coincides with the onset of intensive poliovirus protein synthesis and the appearance of the activity of poliovirus protease 3C. Indeed, the p65-RelA amino acid sequence contains the recognition site for 3C, and recombinant protein 3C was shown to be capable of proteolytic cleavage of p65-RelA, generating truncated product similar to that observed during poliovirus infection. Cleavage of p65-RelA occurs during replication of ECHO-1 and rhinovirus 14, suggesting that inactivation of NF-kappaB function by proteolytic cleavage of p65-RelA is the common mechanism by which picornaviruses suppress the innate immune response. |
| Databáze: | OpenAIRE |
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