Protective effects of citicoline-containing eye drops against UVB-Induced corneal oxidative damage in a rat model
| Autor: | Ecem Onder Tokuc, Ahmet Ozturk, Selenay Furat Rençber, Yusufhan Yazir, Nurşen Yüksel, Riza Emre Ergun, Gokhan Duruksu |
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| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine Antioxidant Ultraviolet Rays medicine.medical_treatment Thiophenes Pharmacology Cornea Superoxide dismutase 03 medical and health sciences Cellular and Molecular Neuroscience chemistry.chemical_compound 0302 clinical medicine medicine Animals Rats Wistar skin and connective tissue diseases Expectorants integumentary system biology Glutathione Malondialdehyde eye diseases Sensory Systems Rats Vascular endothelial growth factor Disease Models Animal Oxidative Stress Ophthalmology 030104 developmental biology medicine.anatomical_structure chemistry Catalase 030221 ophthalmology & optometry biology.protein sense organs Ophthalmic Solutions Citicoline Corneal Injuries medicine.drug |
| Zdroj: | Experimental Eye Research. 208:108612 |
| ISSN: | 0014-4835 |
| DOI: | 10.1016/j.exer.2021.108612 |
| Popis: | It has been reported that citicoline increases antioxidant activity in some tissues. However, the effect of citicoline on corneal wound-healing has not yet been demonstrated. The aim was to investigate the protective effects of citicoline on ultraviolet B (UVB) radiation–induced corneal oxidative damage in a rat model. Four groups (eight animals each) were investigated: controls; UVB only; UVB/citicoline; and citicoline only. Corneal oxidative damage was induced by exposure to UVB radiation at 560 μW/cm2 for five days in the UVB-exposed groups and 1% citicoline eye drops were applied (3xday) for eight days in the two citicoline groups. Corneal surface damage was evaluated by opacity and fluorescein staining. Corneal injury was assessed biochemically by measuring the concentrations of glutathione (GSH) and malondialdehyde (MDA) and the activity of corneal superoxide dismutase (SOD) and catalase. Matrix metalloproteinase (MMP) −2 and −9 and caspase-3 were evaluated by immunofluorescent staining and microscopic examination and by Western blot analysis. Corneal gene expression analysis was performed for vascular endothelial growth factor (VEGF), interleukin-1 beta (IL-1β) and transforming growth factor-beta (TGF-β). UVB radiation caused significant epithelial damage and evident opacity in the cornea, together with a local decrease in SOD, catalase and GSH activity. Corneal MDA concentrations increased with UVB exposure. The UVB/Citicoline group had significantly less corneal damage, greater SOD, catalase and GSH activity, and decreased MDA concentrations compared to the UVB only group (p |
| Databáze: | OpenAIRE |
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