Flow analysis on microcasting with degassed polydimethylsiloxane micro-channels for cell patterning with cross-linked albumin

Autor: Shunsuke Furutani, Hidenori Nagai, Nobuyuki Tanaka, Takayuki Kawai, Hironori Yamazoe, Yo Tanaka, Yigang Shen
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Cell Culture Techniques
02 engineering and technology
medicine.disease_cause
Pathology and Laboratory Medicine
01 natural sciences
Biochemistry
Physical Chemistry
Sepharose
Myoblasts
chemistry.chemical_compound
Viscosity
Mice
Materials Physics
Medicine and Health Sciences
Dehydration (Medicine)
Microstructure
Flow Rate
Multidisciplinary
Aqueous solution
Physics
Classical Mechanics
021001 nanoscience & nanotechnology
Volumetric flow rate
Chemistry
Physical Sciences
Metallurgy
Agarose
Medicine
Microtechnology
Biological Cultures
0210 nano-technology
Research Article
Materials science
Science
Materials Science
Fluid Mechanics
010402 general chemistry
Research and Analysis Methods
Continuum Mechanics
Signs and Symptoms
Castings
Diagnostic Medicine
Mold
Albumins
medicine
Animals
Dimethylpolysiloxanes
Polydimethylsiloxane
technology
industry
and agriculture
Biology and Life Sciences
Proteins
Fluid Dynamics
Cell Cultures
0104 chemical sciences
chemistry
Chemical engineering
Chemical Properties
Zdroj: PLoS ONE
PLoS ONE, Vol 15, Iss 5, p e0232518 (2020)
ISSN: 1932-6203
Popis: Patterned cell culturing is one of the most useful techniques for understanding the interaction between geometric conditions surrounding cells and their behaviors. The authors previously proposed a simple method for cell patterning with an agarose gel microstructure fabricated by microcasting with a degassed polydimethylsiloxane (PDMS) mold. Although the vacuum pressure produced from the degassed PDMS can drive a highly viscous agarose solution, the influence of solution viscosity on the casting process is unknown. This study investigated the influences of micro-channel dimensions or solution viscosity on the flow of the solution in a micro-channel of a PDMS mold by both experiments and numerical simulation. It was found experimentally that the degassed PDMS mold was able to drive a solution with a viscosity under 575 mPa·s. A simulation model was developed which can well estimate the flow rate in various dimensions of micro-channels. Cross-linked albumin has low viscosity (1 mPa·s) in aqueous solution and can undergo a one-way dehydration process from solution to solid that produces cellular repellency after dehydration. A microstructure of cross-linked albumin was fabricated on a cell culture dish by the microcasting method. After cells were seeded and cultivated on the cell culture dish with the microstructure for 7 days, the cellular pattern of mouse skeletal myoblast cell line C2C12 was observed. The microcasting with cross-linked albumin solution enables preparation of patterned cell culture systems more quickly in comparison with the previous agarose gel casting, which requires a gelation process before the dehydration process.
Databáze: OpenAIRE
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