Characterization of a Novel Cytochrome Involved in Geobacter sulfurreducens' Electron Harvesting Pathways

Autor:	Liliana R. Teixeira, Tomás M. Fernandes, Marta A. Silva, Leonor Morgado, Carlos A. Salgueiro
Rok vydání:	2022
Předmět:	Electron Transport Bacterial Proteins Organic Chemistry Cytochromes Electrons General Chemistry Heme Ligands Oxidation-Reduction Catalysis
Zdroj:	Chemistry (Weinheim an der Bergstrasse, Germany). 28(66)
ISSN:	1521-3765
Popis:	Electron harvesting bacteria are key targets to develop microbial electrosynthesis technologies, which are valid alternatives for the production of value-added compounds without utilization of fossil fuels. Geobacter sulfurreducens, that is capable of donating and accepting electrons from electrodes, is one of the most promising electroactive bacteria. Its electron transfer mechanisms to electrodes have been progressively elucidated, however the electron harvesting pathways are still poorly understood. Previous studies showed that the periplasmic cytochromes PccH and GSU2515 are overexpressed in current-consuming G. sulfurreducens biofilms. PccH was characterized, though no putative partners have been identified. In this work, GSU2515 was characterized by complementary biophysical techniques and in silico simulations using the AlphaFold neural network. GSU2515 is a low-spin monoheme cytochrome with a disordered N-terminal region and an α-helical C-terminal domain harboring the heme group. The cytochrome undergoes a redox-linked heme axial ligand switch, with Met
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f16ac116da9ab3980cd2b9318e8a2e90 https://pubmed.ncbi.nlm.nih.gov/36037397 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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