Microparticles released by Listeria monocytogenes-infected macrophages are required for dendritic cell-elicited protective immunity
| Autor: | Bo Huang, Zhuoshun Yang, Geming Lu, Ruihua Zhang, Pingwei Xu, Huabao Xiong, Miao Cui, Huafeng Zhang, Wenqian Cai, Jing Liu, Yi Zhang, Huang-Zhong Shi, Reto A. Schwendener |
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| Přispěvatelé: | University of Zurich, Huang, Bo |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
T-Lymphocytes
Immunology Antigen presentation chemical and pharmacologic phenomena Microbiology Mice Immune system Antigen Immunity Cell-Derived Microparticles MHC class I Immunology and Allergy Animals Listeriosis Cells Cultured Cell Proliferation 2403 Immunology Antigen Presentation Antigens Bacterial Mice Inbred BALB C biology Macrophages 10061 Institute of Molecular Cancer Research Cell Differentiation Dendritic cell 2725 Infectious Diseases Dendritic Cells Actin cytoskeleton Listeria monocytogenes Mice Inbred C57BL Actin Cytoskeleton Infectious Diseases biology.protein 2723 Immunology and Allergy 570 Life sciences Bacterial antigen Research Article Subcellular Fractions |
| Popis: | Interplay between macrophages and dendritic cells in the processing and presentation of bacterial antigens for T-cell immune responses remains poorly understood. Using a Listeria monocytogenes (Lm) infection model, we demonstrate that dendritic cells (DCs) require the support of macrophages to elicit protective immunity against Lm infection. DCs themselves were inefficient at taking up Lm but capable of taking up microparticles (MPs) released by Lm-infected macrophages. These MPs transferred Lm antigens to DCs, allowing DCs to present Lm antigen to effector T cells. MP-mediated Lm antigen transfer required MHC class I participation, since MHC class I deficiency in macrophages resulted in a significant reduction of T-cell activation. Moreover, the vaccination of mice with MPs from Lm-infected macrophages produced strong protective immunity against Lm infection. We here identify an intrinsic antigen transfer program between macrophages and DCs during Lm infection, and emphasize that macrophages also play an essential role in DC-elicited Lm-specific T-cell responses. |
| Databáze: | OpenAIRE |
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