Visualization of Neutrophil Extracellular Traps and Fibrin Meshwork in Human Fibrinopurulent Inflammatory Lesions: III. Correlative Light and Electron Microscopic Study
| Autor: | Kazuya Shiogama, Yutaka Tsutsumi, Yasuyoshi Mizutani, Takanori Onouchi, Takashi Takaki |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Fibrinogen-gamma chain Histology Physiology Scanning electron microscope neutrophil extracellular traps Inflammation Fibril Biochemistry Fibrin Pathology and Forensic Medicine 03 medical and health sciences chemistry.chemical_compound medicine fibrin correlative light and electron microscopy DAPI confocal laser scanning microscopy biology Chemistry Lactoferrin Regular Article Cell Biology Neutrophil extracellular traps Cell biology 030104 developmental biology biology.protein Biophysics medicine.symptom scanning electron microscopy |
| Zdroj: | Acta Histochemica et Cytochemica |
| ISSN: | 1347-5800 0044-5991 |
| DOI: | 10.1267/ahc.16028 |
| Popis: | Neutrophil extracellular traps (NETs) released from dead neutrophils at the site of inflammation represent webs of neutrophilic DNA stretches dotted with granule-derived antimicrobial proteins, including lactoferrin, and play important roles in innate immunity against microbial infection. We have shown the coexistence of NETs and fibrin meshwork in varied fibrinopurulent inflammatory lesions at both light and electron microscopic levels. In the present study, correlative light and electron microscopy (CLEM) employing confocal laser scanning microscopy and scanning electron microscopy was performed to bridge light and electron microscopic images of NETs and fibrin fibrils in formalin-fixed, paraffin-embedded, autopsied lung sections of legionnaire’s pneumonia. Lactoferrin immunoreactivity and 4'-6-diamidino-2-phenylindole (DAPI) reactivity were used as markers of NETs, and fibrin was probed by fibrinogen gamma chain. Of note is that NETs light microscopically represented as lactoferrin and DAPI-colocalized dots, 2.5 μm in diameter. CLEM gave super-resolution images of NETs and fibrin fibrils: “Dotted” NETs were ultrastructurally composed of fine filaments and masses of 58 nm-sized globular materials. A fibrin fibril consisted of clusters of smooth-surfaced filaments. NETs filaments (26 nm in diameter) were significantly thinner than fibrin filaments (295 nm in diameter). Of note is that CLEM was applicable to formalin-fixed, paraffin-embedded sections of autopsy material. |
| Databáze: | OpenAIRE |
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