Differential gene expression in Mycobacterium bovis challenged monocyte-derived macrophages of cattle
| Autor: | Rehan Khan, Sarvjeet, Ajay Vir Singh, Sanjeev Kumar Shukla, Lakshya Veer Singh, Anuj Ahuja, Anuj Chauhan, Chandan Prakash, Naveen Sharma, Shubhra Shukla, Manjit Panigrahi |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Chemokine CXCL6 Microarray 030106 microbiology Down-Regulation Tuberculin Microbiology 03 medical and health sciences Multiplicity of infection Immune system Gene expression Animals RNA Messenger Gene Cell Proliferation Mycobacterium bovis biology Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Macrophages Interleukin-8 Receptor Adenosine A3 NF-kappa B p50 Subunit biology.organism_classification Molecular biology Toll-Like Receptor 2 Up-Regulation TLR2 030104 developmental biology Infectious Diseases Gene Expression Regulation Host-Pathogen Interactions B7-1 Antigen Leukocytes Mononuclear Cattle Female Tuberculosis Bovine Functional genomics Biomarkers Transcription Factors |
| Zdroj: | Microbial Pathogenesis. 113:480-489 |
| ISSN: | 0882-4010 |
| Popis: | A functional genomics approach was used to examine the immune response for transcriptional profiling of PBMC M. bovis infected cattle and healthy control cattle to stimulation with bovine tuberculin (purified protein derivative PPD-b). Total cellular RNA was extracted from non-challenged control and M. bovis challenged MDM for all animals at intervals of 6 h post-challenge, in response to in-vitro challenge with M. bovis (multiplicity of infection 2:1) and prepared for global gene expression analysis using the Agilent Bovine (V2) Gene Expression Microarray, 8 × 60 K. The pattern of expression of these genes in PPD bovine stimulated PBMC provides the first description of an M. bovis specific signature of infection that may provide insights into the molecular basis of the host response to infection. Analysis of these mapped reads showed 2450 genes (1291 up regulated and 1158 down regulated) 462 putative natural antisense transcripts (354 up-regulated and 108 down regulated) that were differentially expressed based on sense and antisense strand data, respectively (adjusted P-value ≤ 0.05). The results provided enrichment for genes involved top ten up regulated and down regulated panel of genes, including transcription factors proliferation of T and B lymphocytes. The highest differentially-expressed genes were associated to immune and inflammatory responses, immunity, differentiation, cell growth, apoptosis, cellular trafficking and regulation of lipolysis and thermogenesis. Microarray results were confirmed in infected cattle by RT qPCR to identify potential biomarkers TLR2, CD80, NFKB1, IL8, CXCL6 and ADORA3 of bovine tuberculosis. |
| Databáze: | OpenAIRE |
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