Ex vivo susceptibility of Plasmodium falciparum isolates from Dakar, Senegal, to seven standard anti-malarial drugs
| Autor: | P. S. Mbaye, Silmane Diawara, F. Fall, Yaya Diémé, Bakary Diatta, Raymond Bercion, Eric Baret, Boubacar Wade, Khadidiatou Ba Fall, Christophe Rogier, Kowry Sow, Bécaye Fall, Bruno Pradines |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
lcsh:Arctic medicine. Tropical medicine
lcsh:RC955-962 medicine.medical_treatment Plasmodium falciparum Protozoan Proteins Dihydroartemisinin Enzyme-Linked Immunosorbent Assay Pharmacology Lumefantrine lcsh:Infectious and parasitic diseases chemistry.chemical_compound Antimalarials Inhibitory Concentration 50 Parasitic Sensitivity Tests Chloroquine parasitic diseases medicine Humans lcsh:RC109-216 Artemisinin Malaria Falciparum Quinine biology L-Lactate Dehydrogenase Mefloquine Research biology.organism_classification Senegal Infectious Diseases chemistry Parasitology Ex vivo medicine.drug |
| Zdroj: | Malaria Journal, Vol 10, Iss 1, p 310 (2011) Malaria Journal |
| ISSN: | 1475-2875 |
| Popis: | Background As a result of widespread chloroquine and sulphadoxine-pyrimethamine resistance, artemisinin-based combination therapy (ACT) (which includes artemether-lumefantrine and artesunate-amodiaquine) has been recommended as a first-line anti-malarial regimen in Senegal since 2006. Since then, there have been very few reports on the ex vivo susceptibility of Plasmodium falciparum to anti-malarial drugs. To examine whether parasite susceptibility has been affected by the widespread use of ACT, the ex vivo susceptibility of local isolates was assessed at the military hospital of Dakar. Methods The ex vivo susceptibility of 93 P. falciparum isolates from Dakar was successfully determined using the Plasmodium lactate dehydrogenase (pLDH) ELISA for the following drugs: chloroquine (CQ), quinine (QN), mefloquine (MQ), monodesethylamodiaquine (MDAQ), lumefantrine (LMF), dihydroartemisinin (DHA) and doxycycline (DOX). Results After transformation of the isolate IC50 in ratio of IC50 according to the susceptibility of the 3D7 reference strain (isolate IC50/3D7 IC50), the prevalence of the in vitro resistant isolates with reduced susceptibility was 50% for MQ, 22% for CQ, 12% for DOX, 6% for both QN and MDAQ and 1% for the drugs LMF and DHA. The highest significant positive correlations were shown between responses to CQ and MDAQ (r = 0.569; P < 0.0001), LMF and QN (r = 0.511; P < 0.0001), LMF and DHA (r = 0.428; P = 0.0001), LMF and MQ (r = 0.413; P = 0.0002), QN and DHA (r = 0.402; P = 0.0003) and QN and MQ (r = 0.421; P = 0.0001). Conclusions The introduction of ACT in 2002 has not induced a decrease in P. falciparum susceptibility to the drugs DHA, MDAQ and LMF, which are common ACT components. However, the prevalence of P. falciparum isolates with reduced susceptibility has increased for both MQ and DOX. Taken together, these data suggest that intensive surveillance of the P. falciparum in vitro susceptibility to anti-malarial drugs in Senegal is required. |
| Databáze: | OpenAIRE |
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