A neurokinin 1 receptor antagonist decreases adhesion reformation after laparoscopic lysis of adhesions in a rat model of adhesion formation

Autor: Arthur F. Stucchi, R. Matteotti, James M. Becker, Scott G. Prushik, Cary B. Aarons, Karen L. Reed, Adam C. Gower, Susan E. Leeman
Rok vydání: 2007
Předmět:
Zdroj: Surgical Endoscopy. 21:1790-1795
ISSN: 1432-2218
0930-2794
DOI: 10.1007/s00464-007-9248-2
Popis: Up to 94% of patients experience fibrous adhesions after abdominal surgery, and a significant number of these patients require a second operation for open or laparoscopic lysis of adhesions (LOA). The authors have previously shown that inhibition of the binding of tachykinin ligands to the neurokinin 1 receptor (NK-1R) using the neurokinin 1 receptor antagonist (NK-1RA) CJ-12,255 decreases primary adhesion formation and upregulates the peritoneal fibrinolytic system in a rat model. Whereas most studies have focused on the prevention of primary adhesions, few have addressed adhesion reformation after LOA. This study aimed to determine the effects of NK-1RA administration on adhesion reformation and peritoneal fibrinolytic activity after laparoscopic LOA. Adhesions were induced in 31 rats using our previously described ischemic button model. The rats underwent laparoscopy 7 days later, during which adhesions were scored and lysed followed by administration of the NK-1RA or saline. Then 7 days after LOA, 23 rats were killed and adhesions were scored. Eight rats also were killed 24 h after the LOA to obtain peritoneal tissue and fluid, which were analyzed for tissue plasminogen activator (tPA) mRNA expression and peritoneal fibrinolytic activity by reverse transcriptase-polymerase chain reaction (RT-PCR) and bioassay, respectively. At laparoscopy, 79% ± 3% of the buttons formed adhesions. In the saline-administered control animals, 42% ± 3.2% of the buttons reformed adhesions after LOA (p
Databáze: OpenAIRE
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