Effect of Freezing Treatment on Colostrum to Prevent the Transmission of Bovine Leukemia Virus
| Autor: | Satoshi Tachibana, Yasuhiro Oue, Yasuhiro Konno, Toru Kanno, Hiroki Edamatsu, Shinichi Hatama, Kenji Murakami, Ryoko Ishihara |
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| Rok vydání: | 2014 |
| Předmět: |
viruses
animal diseases Cattle Diseases Biology fluids and secretions Virology Freezing Leukemia Virus Bovine Leukocytes Animals Bioassay sheep bioassay Infectivity Sheep General Veterinary Bovine leukemia virus Transmission (medicine) Inoculation Colostrum virus diseases food and beverages Enzootic Bovine Leukosis Note biology.organism_classification bovine leukemia virus freezing treatment Cattle |
| Zdroj: | The Journal of Veterinary Medical Science |
| ISSN: | 1347-7439 0916-7250 |
| Popis: | Here, we used a sheep bioassay to determine the effect of freezing colostrum to prevent the transmission of bovine leukemia virus (BLV) among neonatal calves. Leukocytes were isolated from the colostrum of a BLV-infected Holstein cow and were then either left untreated (control) or freeze-thawed. A sheep inoculated intraperitoneally with the untreated leukocytes was infected with BLV at 3 weeks after inoculation, whereas the sheep inoculated with treated leukocytes did not become infected. The uninfected sheep was inoculated again with leukocytes isolated from the colostrum of another BLV-infected Holstein cow after freezing treatment, and again it did not become infected with BLV. Finally, this sheep was inoculated with the leukocytes isolated from the colostrum of another virus-infected cow without freezing treatment, and it became infected with BLV at 4 weeks after inoculation. The results indicate that colostrum should be frozen as a useful means of inactivating the infectivity of BLV-infected lymphocytes. |
| Databáze: | OpenAIRE |
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