Transcriptional regulation of the interferon-gamma-inducible tryptophanyl-tRNA synthetase includes alternative splicing
| Autor: | Anette Bejder, Jan Fleckner, Just Justesen, Anne B. Tolstrup |
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| Rok vydání: | 1995 |
| Předmět: |
Transcription
Genetic Molecular Sequence Data Tryptophan-tRNA Ligase Biology Transfection Biochemistry Exon Interferon-gamma Transcriptional regulation Humans RNA Messenger Cloning Molecular Promoter Regions Genetic Molecular Biology Gene Regulation of gene expression Reporter gene Messenger RNA Base Sequence Alternative splicing Promoter Cell Biology Alkaline Phosphatase Molecular biology Alternative Splicing Oligodeoxyribonucleotides Enzyme Induction HeLa Cells |
| Zdroj: | The Journal of biological chemistry. 270(1) |
| ISSN: | 0021-9258 |
| Popis: | We have investigated the transcriptional control elements of the human interferon (IFN)-gamma-induced tryptophanyl-tRNA synthetase (hWRS) gene and characterized the transcripts. Transcription leads to a series of mRNAs with different combinations of the first exons. The full-length mRNA codes for a 55-kDa protein (hWRS), but a mRNA lacking exon II is present in almost as high amounts as the full-length transcript. This alternatively spliced mRNA is probably translated into a 48-kDa protein starting from Met48 in exon III. The predicted 48-kDa protein corresponds exactly to an IFN-gamma-inducible protein previously detected by two-dimensional gel electrophoresis. By isolation of genomic clones and construction of plasmids containing hWRS promoter fragments fused to the secreted alkaline phosphatase reporter gene we have mapped a promoter region essential for IFN-mediated gene activation. This region contains IFN-stimulated response elements (ISRE) as well as a Y-box and a gamma-activated sequence (GAS) element. IFN-gamma inducibility of hWRS depends on ongoing protein synthesis, suggesting that so far undescribed transcription factors apart from the latent GAS-binding protein p91 contribute to gene activation. This could be interferon-regulatory factor-1, which binds ISRE elements. |
| Databáze: | OpenAIRE |
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