Dietary iron variably modulates assembly of the intestinal microbiota in colitis-resistant and colitis-susceptible mice
| Autor: | Ian M. Carroll, Janelle C. Arthur, S Plevy, Nitsan Maharshak, Cory Brouwer, Ernesto Perez-Chanona, Anthony A. Fodor, Raad Z. Gharaibeh, R. Balfour Sartor, Christian Jobin, Melissa Ellermann |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
colitis gut microbiome medicine.disease_cause Mice 0302 clinical medicine 2. Zero hunger Dietary iron biology Gastroenterology Iron deficiency Enterobacteriaceae Interleukin-10 Intestines Infectious Diseases Role of gut microbiome in GI disease 030211 gastroenterology & hepatology Disease Susceptibility medicine.symptom Iron Dietary Microbiology (medical) Colon IBD Animal models of GI-diseases with microbial components Inflammation Mice Transgenic Microbiology Defining/profiling gut microbiome 03 medical and health sciences medicine Escherichia coli Animals Genetic Predisposition to Disease lcsh:RC799-869 Colitis Wild type biology.organism_classification medicine.disease Inflammatory Bowel Diseases Gastrointestinal Microbiome Disease Models Animal 030104 developmental biology Research Paper/Report Dysbiosis lcsh:Diseases of the digestive system. Gastroenterology Bacteria |
| Zdroj: | Gut Microbes Gut Microbes, Vol 11, Iss 1, Pp 32-50 (2020) |
| ISSN: | 1949-0984 1949-0976 |
| Popis: | Iron deficiency, a common comorbidity of gastrointestinal inflammatory disorders such as inflammatory bowel diseases (IBD), is often treated with oral iron supplementation. However, the safety of oral iron supplementation remains controversial because of its association with exacerbated disease activity in a subset of IBD patients. Because iron modulates bacterial growth and function, one possible mechanism by which iron may exacerbate inflammation in susceptible hosts is by modulating the intestinal microbiota. We, therefore, investigated the impact of dietary iron on the intestinal microbiota, utilizing the conventionalization of germ-free mice as a model of a microbial community in compositional flux to recapitulate the instability of the IBD-associated intestinal microbiota. Our findings demonstrate that altering intestinal iron availability during community assembly modulated the microbiota in non-inflamed wild type (WT) and colitis-susceptible interleukin-10-deficient (Il10−/-) mice. Depletion of luminal iron availability promoted luminal compositional changes associated with dysbiotic states irrespective of host genotype, including an expansion of Enterobacteriaceae such as Escherichia coli. Mechanistic in vitro growth competitions confirmed that high-affinity iron acquisition systems in E. coli enhance its abundance over other bacteria in iron-restricted conditions, thereby enabling pathobiont iron scavenging during dietary iron restriction. In contrast, distinct luminal community assembly was observed with dietary iron supplementation in WT versus Il10−/- mice, suggesting that the effects of increased iron on the microbiota differ with host inflammation status. Taken together, shifts in dietary iron intake during community assembly modulate the ecological structure of the intestinal microbiota and is dependent on host genotype and inflammation status. |
| Databáze: | OpenAIRE |
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