Sodium glycididazole enhances the radiosensitivity of laryngeal cancer cells through downregulation of ATM signaling pathway
| Autor: | Rong Wu, Rui Xing, Yu Nan Sun, Guo-Liang Fan, Hong Mei Wang, Nan Niu, Feng Chi, Yan Xin, Yue Can Zeng, Qiong Yu Duan, Ming Xue, Jing Zeng |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Radiation-Sensitizing Agents Cell cycle checkpoint DNA Repair DNA repair DNA damage Cell Survival Apoptosis Ataxia Telangiectasia Mutated Proteins Biology 03 medical and health sciences Mice 0302 clinical medicine Cell Line Tumor medicine Biomarkers Tumor Sodium Glycididazole Animals Humans Radiosensitivity Hypoxia Laryngeal Neoplasms Cell Cycle Imidazoles General Medicine Hep G2 Cells Cell cycle Xenograft Model Antitumor Assays Tumor Burden Disease Models Animal 030104 developmental biology 030220 oncology & carcinogenesis Cancer cell Cancer research Signal Transduction |
| Zdroj: | Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 37(5) |
| ISSN: | 1423-0380 |
| Popis: | The purpose of this study was to evaluate the radiation-enhancing effect of sodium glycididazole, and the corresponding mechanisms of action on laryngeal cancer cells. Two laryngeal cancer cell lines (Hep-2 and UT-SCC-19A) were irradiated with X-rays in the presence or absence of sodium glycididazole. Cell survival, DNA damage and repair, cell apoptosis, cell cycle distribution, expression of proteins related to cell cycle checkpoint, and apoptosis were measured. Significantly increased DNA damages, decreased cells in the G1 phase, arrested cells at G2/M phase, decreased DNA repair protein XRCC1 foci formation, and enhanced cell apoptosis were observed in laryngeal cell lines treated by sodium glycididazole combined with irradiation compared with the irradiation alone. The combined treatment downregulated the protein expressions of ataxia-telangiectasia mutated (ATM), p-ATM, CHK2, and P53 but upregulated the protein expressions of MDM2 and Cdk2. This study indicates that sodium glycididazole enhances the radiosensitivity of laryngeal cancer cells through downregulation of ATM signaling pathway in vitro and in vivo. |
| Databáze: | OpenAIRE |
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