Influence of Stem Cell Therapy on Thyroid Function and Reactive Oxygen Species Production in Diabetic Rats
| Autor: | Gustavo Monnerat Cahil, Mariana Lopes de Freitas, Diorney Luiz Souza Gran da Silva, Vânia Maria Corrêa da Costa, Vitória Santório de São José, Felippe Mousovich Neto, Denise P. Carvalho, Ricardo Cardoso, Andrea Claudia Freitas Ferreira, Emiliano Medei, Rodrigo S. Fortunato |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Thyroid Hormones Endocrinology Diabetes and Metabolism Clinical Biochemistry Population Cell- and Tissue-Based Therapy Thyroid Gland 030209 endocrinology & metabolism Thyroid Function Tests Mesenchymal Stem Cell Transplantation Biochemistry Thyroid function tests Diabetes Mellitus Experimental 03 medical and health sciences 0302 clinical medicine Endocrinology Thyroid peroxidase Internal medicine medicine Animals Rats Wistar education education.field_of_study biology medicine.diagnostic_test business.industry Thyroid disease Biochemistry (medical) Thyroid Mesenchymal Stem Cells General Medicine Streptozotocin medicine.disease Rats 030104 developmental biology medicine.anatomical_structure biology.protein Thyroid function business Reactive Oxygen Species medicine.drug Hormone |
| Zdroj: | Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme. 50(4) |
| ISSN: | 1439-4286 |
| Popis: | Cell therapy with mesenchymal stem cells (MSC) has been proposed for the treatment of diabetes mellitus (DM). It is known that the prevalence of thyroid disease is higher among diabetic patients than in general population. Therefore, our aim was to investigate the effect of the treatment with MSC on thyroid function and ROS generation in an experimental model of type 1 DM. Adult male Wistar rats were divided into the following groups: control, DM (80 mg/kg BW streptozotocin, iv.) and DM+MSC. MSC treatment occurred 4 weeks after DM induction and the animals were euthanized 4 weeks after MSC administration. We also evaluated the effect of co-culture with MSC or extracellular vesicles (EV) obtained from these cells on the rat thyroid cell line PCCL3 exposed to high glucose. Thyroid H2O2 generation was increased in DM, which was reversed by MSC treatment. These changes paralled a significant DuOx1 mRNA increase. The incubation of PCCL3 with high glucose increased extracellular H2O2 generation, which was reversed by both the co-culture with MSC and EV. Even though MSC treatment normalized thyroid ROS generation, serum thyroid hormone (TH) concentration remained low, along with increased serum TSH concentrations. Thyroperoxidase (TPO) activity, was reduced in DM, and MSC treatment did not normalize TPO. Therefore, we conclude that the treatment with MSC was able to reverse the increased thyroid H2O2 generation in diabetic animals and in PCCL3 cells exposed to high glucose, an effect probably mediated by EV produced by these cells, acting in a paracrine fashion. |
| Databáze: | OpenAIRE |
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