Molecular characterization of endangered endemic plant Aloe pseudorubroviolacea using chloroplast matK and plastid rbcL gene
| Autor: | Anis Ahamed, Rahmah N. Al Qthanin, Ibrahim A. Arif, Annamalai Panneerselvam, Abdul Wali Al-Khulaidi, Abdullah Alaklabi |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Sequence analysis ved/biology.organism_classification_rank.species Rare species Endangered species Biology 01 natural sciences Homology (biology) maturase K 03 medical and health sciences Aloe pseudorubroviolacea Botany Asphodelaceae Maturase K lcsh:QH301-705.5 Gene ved/biology 5-biphosphate carboxylase biology.organism_classification Homology Ribulose-1 Chrysopogon gryllus 030104 developmental biology lcsh:Biology (General) Similar Original Article General Agricultural and Biological Sciences 010606 plant biology & botany |
| Zdroj: | Saudi Journal of Biological Sciences, Vol 28, Iss 1, Pp 1123-1127 (2021) Saudi Journal of Biological Sciences |
| ISSN: | 1319-562X |
| DOI: | 10.1016/j.sjbs.2020.11.042 |
| Popis: | An endangered and rare species Aloe pseudorubroviolacea from the plant family Asphodelaceae which is presently recorded as endangered in Saudi Arabia collected from Al-Baha region of Saudi Arabia its GPS Latitude and Longitude coordinates 19.8345, 41.5481. The chloroplast matK and rbcL gene was considered in this study based on molecular identification the size is about 571 and 664 bp respectively. From the sequence analysis the gene matK and rbcL confirm that this species is very much closely related with A. rubroviolacea and also inter related with the species Astroloba rubriflora, Chrysopogon gryllus, Chortolirion angolense shows about 98.7% sequence homology. The partial matK and rbcL gene sequence discriminate Aloe pseudorubroviolacea from the closely related plant species, A. rubroviolacea. The gene sequence of rbcL discriminates the species from Chrysopogon gryllus and Chortolirion angolense, demonstrates the nucleotide variations in 3 different sites (623C/T; 653C/T; 700C/A). This study showed that matK and rbcL sequence region of chloroplast gene used to authenticate the samples of A. pseudorubroviolacea and which provide to help in correct identification and conservation process of this medicinally valuable endangered plant species. |
| Databáze: | OpenAIRE |
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