A ubiquitin C-terminal hydrolase is required to maintain osmotic balance and execute actin-dependent processes in the early C. elegans embryo
| Autor: | Ralf Schnabel, Susanne Kaitna, Michael Glotzer, Anthony A. Hyman, Heinke Schnabel |
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| Rok vydání: | 2002 |
| Předmět: |
Embryo
Nonmammalian Mutant Molecular Sequence Data Ubiquitin C-Terminal Hydrolase Cell polarity Animals Cleavage furrow Amino Acid Sequence Caenorhabditis elegans Caenorhabditis elegans Proteins Actin Cells Cultured Cell Size biology Base Sequence Ubiquitin Cell Polarity Chromosome Mapping Cell Biology Water-Electrolyte Balance biology.organism_classification Actin cytoskeleton Cell biology Culture Media Actin Cytoskeleton Hypotonic Solutions Mutation Stress Mechanical Thiolester Hydrolases Ubiquitin Thiolesterase Cytokinesis Cell Division |
| Zdroj: | Journal of cell science. 115(Pt 11) |
| ISSN: | 0021-9533 |
| Popis: | In the early Caenorhabditis elegans embryo, establishment of cell polarity and cytokinesis are both dependent upon reorganization of the actin cytoskeleton. Mutations in the cyk-3 gene cause maternal effect embryonic lethality. Embryos produced by homozygous cyk-3 mutant animals become multinucleate. We have further analyzed the cyk-3mutant phenotype and have found that cyk-3 mutant embryos fail to properly polarize the actin cytoskeleton and fail to segregate germline determinants. In addition, they fail to assemble an intact cleavage furrow. However, we have found that cyk-3 mutant embryos are intrinsically defective in osmotic regulation and that the cytokinesis defects can be partially rescued by providing osmotic support. The cyk-3 gene has been identified and found to encode a ubiquitin C-terminal hydrolase that is active against model substrates. These data indicate that the deubiquitination of certain substrates by CYK-3 is crucial for cellular osmoregulation. Defects in osmoregulation appear to indirectly affect actin-dependent processes. |
| Databáze: | OpenAIRE |
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