Isolation and Sequence Analysis of the Rat Dihydrolipoamide Succinyltransferase Gene
| Autor: | Kyoko Nakano, Masako Shimura, Shihoko Suzuki, Shigeo Ohta, Sadayuki Matuda, Shiro Nakagawa, Mariko Tanabe |
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| Rok vydání: | 2002 |
| Předmět: |
Sequence analysis
TATA box Molecular Sequence Data Biology Biochemistry Exon Endocrinology Genetics Homologous chromosome Animals Humans Nucleotide Rats Wistar Promoter Regions Genetic Molecular Biology Gene chemistry.chemical_classification Base Sequence Intron Sequence Analysis DNA Molecular biology Rats genomic DNA chemistry Transcription Initiation Site Acyltransferases |
| Zdroj: | DNA Sequence. 13:363-367 |
| ISSN: | 1042-5179 |
| DOI: | 10.1080/1042517021000060777 |
| Popis: | The dihydrolipoamide succinyltransferase (DLST) gene of the f -ketoglutarate dehydrogenase complex ( f -KGDC) was isolated from a rat genomic DNA library and sequenced. This gene was composed of 15 exons and 14 introns like the human DLST gene. Sequence analysis of the promoter-regulatory region of the rat DLST gene- ( Dlst ) showed the possible presence of a CAAT box-sequence and of the sequences for an AP-2 site and three Sp1 sites, but no TATA box-sequence was evidenced. The nucleotide sequences of introns 1 and 4 of the rat Dlst were significantly homologous to those of introns 1 and 4 of the human DLST gene. The sequence analysis of the rat Dlst suggested that the exon coding for the E3- and/or E1-binding domain may have been lost from the gene during evolution in eukaryotic DLST, possibly after mitochondrial symbiosis because prokaryotic DLST possesses the E3- and/or E1-binding domain. |
| Databáze: | OpenAIRE |
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