High within-host diversity found from direct genotyping on post-mortem tuberculosis specimens in a high-burden setting
| Autor: | Alberto L. García-Basteiro, Natalia Rakislova, Anelsio Cossa, Lucilia Lovane, Miguel J. Martínez, Dercio Jordao, Juan Carlos Hurtado, Cesaltina Lorenzoni, Jaume Ordi, Cristina Rodríguez-Grande, Carla Carrilho, Isaac Casas, Clara Menéndez, Mireia Navarro, Fabiola Fernandes, Patricia Muñoz, Laura Pérez-Lago, Paola Castillo, Mamudo R. Ismail, Quique Bassat, Sandra Rodríguez-Maus, Inacio Mandomando, Darío García de Viedma |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Tuberculosis Genotype 030106 microbiology Minisatellite Repeats Biology Mycobacterium tuberculosis 03 medical and health sciences 0302 clinical medicine Bacterial genetics Biopsy medicine Humans 030212 general & internal medicine Allele Genotyping Alleles Mozambique Anàlisi Clonal diversity Genètica bacteriana medicine.diagnostic_test Host (biology) Assaying General Medicine medicine.disease biology.organism_classification Virology East Africa Infectious Diseases Àfrica oriental Autòpsia Autopsy |
| Zdroj: | Dipòsit Digital de la UB Universidad de Barcelona |
| Popis: | Objectives To characterize the clonal complexity in Mycobacterium tuberculosis (MTB) infections considering factors that help maximize the detection of coexisting strains/variants. Methods Genotypic analysis by Mycobacterial Interspersed Repetitive-Unit–Variable-Number Tandem-Repeats (MIRU-VNTR) was performed directly on 70 biopsy specimens from two or more different tissues involving 28 tuberculosis cases diagnosed post-mortem in Mozambique, a country with a high tuberculosis burden. Results Genotypic data from isolates collected from two or more tissues were obtained for 23 of the 28 cases (82.1%), allowing the analysis of within-patient diversity. MIRU-VNTR analysis revealed clonal diversity in ten cases (35.7%). Five cases showed allelic differences in three or more loci, suggesting mixed infection with two different strains. In half of the cases showing within-host diversity, one of the specimens associated with clonal heterogeneity was brain tissue. Conclusions Direct MTB genotyping from post-mortem tissue samples revealed a frequent within-host Mycobacterium tuberculosis diversity, including mixed and polyclonal infections. Most of this diversity would have been overlooked if only standard analysis of respiratory specimens had been performed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|