Isocratic reverse-phase HPLC separation and RIA used in the analysis of neuropeptides in brain tissue
| Autor: | Michael D. Taylor, Peter Jenner, M. L. de Ceballos |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Male
Formic acid Enkephalin Methionine Radioimmunoassay Neuropeptide Peptide Brain tissue Substance P High-performance liquid chromatography Sincalide Cellular and Molecular Neuroscience chemistry.chemical_compound Endocrinology Animals Volatile solvents Chromatography High Pressure Liquid Neurotensin chemistry.chemical_classification Brain Chemistry Chromatography Endocrine and Autonomic Systems Sulfates Neuropeptides Rats Inbred Strains General Medicine Reversed-phase chromatography Rats Neurology chemistry hormones hormone substitutes and hormone antagonists Enkephalin Leucine |
| Zdroj: | Neuropeptides. 20(3) |
| ISSN: | 0143-4179 |
| Popis: | A reverse-phase, high-performance liquid chromatographic (HPLC) method was employed to separate and characterise five neuropeptides from complex mixtures, with important advantages over methods employed earlier using combined HPLC-RIA studies. Peptides were separated using 0.5M pyridine-0.5M formic acid buffer, pH 4, containing propan-l-ol 14% (met-enkephalin, leu-enkephalin, neurotensin) or 20% (CCK-8-S, substance P) at a flow rate of 1.0 ml/min. Isocratic conditions, and volatile solvents, resulted in a highly reproducible method, producing samples in a form designed for subsequent RIA. The application and importance of the procedure is demonstrated by comparison of the measurements of apparent peptide levels in crude brain extracts with those of authentic peptides as determined after HPLC purification. |
| Databáze: | OpenAIRE |
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