Cancer stem cell-derived extracellular vesicles preferentially target MHC-II–macrophages and PD1+ T cells in the tumor microenvironment
| Autor: | Patricia Gonzalez-Callejo, Zihan Guo, Tahereh Ziglari, Natalie Marcia Claudio, Kayla Hoang Nguyen, Naoki Oshimori, Joaquim Seras-Franzoso, Ferdinando Pucci |
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| Přispěvatelé: | Institut Català de la Salut, [Gonzalez-Callejo P] Department of Cell, Developmental & Cancer Biology, Oregon Health and Science University, Portland, Oregon, United States of America. Bionanoplasmonics Group, CIC biomaGUNE, Donostia-San Sebastián, Spain. [Guo Z, Nguyen KH] Department of Cell, Developmental & Cancer Biology, Oregon Health and Science University, Portland, Oregon, United States of America. Program in Biomedical Sciences, Oregon Health and Science University, Portland, Oregon, United States of America. [Ziglari T] Department of Cell, Developmental & Cancer Biology, Oregon Health and Science University, Portland, Oregon, United States of America. [Claudio NM] Department of Cell, Developmental & Cancer Biology, Oregon Health and Science University, Portland, Oregon, United States of America. Department of Otolaryngology-Head and Neck Surgery, Oregon Health and Science University, Portland, Oregon, United States of America. [Oshimori N] Department of Cell, Developmental & Cancer Biology, Oregon Health and Science University, Portland, Oregon, United States of America. Program in Biomedical Sciences, Oregon Health and Science University, Portland, Oregon, United States of America. Department of Otolaryngology-Head and Neck Surgery, Oregon Health and Science University, Portland, Oregon, United States of America. Department of Dermatology, Oregon Health and Science University, Portland, Oregon, United States of America. [Seras-Franzoso J] Grup de Direccionament i Alliberament Farmacològic, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. [Pucci F] Department of Cell, Developmental & Cancer Biology, Oregon Health and Science University, Portland, Oregon, United States of America. Program in Biomedical Sciences, Oregon Health and Science University, Portland, Oregon, United States of America. Department of Otolaryngology-Head and Neck Surgery, Oregon Health and Science University, Portland, Oregon, United States of America, Vall d'Hebron Barcelona Hospital Campus |
| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: |
Coll - Càncer
Multidisciplinary Neoplasms::Neoplasms::Neoplasms by Site::Head and Neck Neoplasms::Squamous Cell Carcinoma of Head and Neck [DISEASES] células::estructuras celulares::espacio extracelular::vesículas extracelulares [ANATOMÍA] Cells::Cellular Structures::Extracellular Space::Extracellular Vesicles [ANATOMY] Cap - Càncer Cèl·lules canceroses neoplasias::neoplasias::neoplasias por localización::neoplasias de cabeza y cuello::carcinoma de células escamosas de cabeza y cuello [ENFERMEDADES] células::células madre::células madre neoplásicas [ANATOMÍA] Cèl·lules mare Cells::Stem Cells::Neoplastic Stem Cells [ANATOMY] |
| Zdroj: | Scientia |
| Popis: | Cèl·lules mare del càncer; Càncers de cap i coll; Macròfags Cancer stem cells; Head and neck cancers; Macrophages Células madre del cáncer; Cánceres de cabeza y cuello; Macrófagos Immunotherapy is an approved treatment option for head and neck squamous cell carcinoma (HNSCC). However, the response rate to immune checkpoint blockade is only 13% for recurrent HNSCC, highlighting the urgent need to better understand tumor-immune interplay, with the ultimate goal of improving patient outcomes. HNSCC present high local recurrence rates and therapy resistance that can be attributed to the presence of cancer stem cells (CSC) within tumors. CSC exhibit singular properties that enable them to avoid immune detection and eradication. How CSC communicate with immune cells and which immune cell types are preferentially found within the CSC niche are still open questions. Here, we used genetic approaches to specifically label CSC-derived extracellular vesicles (EVs) and to perform Sortase-mediated in vivo proximity labeling of CSC niche cells. We identified specific immune cell subsets that were selectively targeted by EVCSC and that were found in the CSC niche. Native EVCSC preferentially targeted MHC-II–macrophages and PD1+ T cells in the tumor microenvironment, which were the same immune cell subsets enriched within the CSC niche. These observations indicate that the use of genetic technologies able to track EVs without in vitro isolation are a valuable tool to unveil the biology of native EVCSC. European Molecular Biology Organization (EMBO):Patricia Gonzalez-Callejo short-term fellowship; V Foundation for Cancer Research (VFCR):Natalie M Claudio,Ferdinando Pucci 2019-012. |
| Databáze: | OpenAIRE |
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