Differentiation of human gingival mesenchymal stem cells into neuronal lineages in 3D bioconjugated injectable protein hydrogel construct for the management of neuronal disorder
| Autor: | Suresh Ranga Rao, Rajasekaran Subbarayan, Gnanamani Arumugam, Murugan Girija Dinesh, Selvaraj Thirupathi Kumara Raja |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Adult Cellular differentiation Clinical Biochemistry Cell Culture Techniques Gingiva Clinical uses of mesenchymal stem cells Embryoid body Biology Stem cell marker Bioinformatics Mesenchymal Stem Cell Transplantation Biochemistry Hydrogel Polyethylene Glycol Dimethacrylate 03 medical and health sciences Young Adult 0302 clinical medicine Neurosphere Humans Regeneration Molecular Biology Stem cell transplantation for articular cartilage repair Neurons Tissue Engineering Tissue Scaffolds Cell Differentiation Mesenchymal Stem Cells Cell biology 030104 developmental biology Phenotype 030220 oncology & carcinogenesis Molecular Medicine Original Article Stem cell Biomarkers Adult stem cell |
| Zdroj: | Experimental & Molecular Medicine |
| ISSN: | 2092-6413 |
| Popis: | The success of regeneration attempt is based on an ideal combination of stem cells, scaffolding and growth factors. Tissue constructs help to maintain stem cells in a required area for a desired time. There is a need for easily obtainable cells, potentially autologous stem cells and a biologically acceptable scaffold for use in humans in different difficult situations. This study aims to address these issues utilizing a unique combination of stem cells from gingiva and a hydrogel scaffold, based on a natural product for regenerative application. Human gingival mesenchymal stem cells (HGMSCs) were, with due induction, differentiated to neuronal lineages to overcome the problems associated with birth tissue-related stem cells. The differentiation potential of neuronal lineages was confirmed with suitable specific markers. The properties of mesenchymal stem cells in encapsulated form were observed to be similar to free cells. The encapsulated cells (3D) were then subjected to differentiation into neuronal lineages with suitable inducers, and the morphology and gene expression of transient cells were analyzed. HGMSCs was differentiated into neuronal lineages as both free and encapsulated forms without any significant differences. The presence of Nissl bodies and the neurite outgrowth confirm the differentiation. The advantages of this new combination appear to make it a promising tissue construct for translational application. |
| Databáze: | OpenAIRE |
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