Molecular detection of extended spectrum β-lactamase genes in Escherichia coli clinical isolates from diarrhoeic children in Kano, Nigeria
| Autor: | Silvia García-Soto, Bashir Muhammad, Nasir Tukur Dabo, Habeeb Kayode Saka, Vicente Lopez-Chavarrias, María Ugarte-Ruiz, Julio Alvarez |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Antibiotics Artificial Gene Amplification and Extension Drug resistance medicine.disease_cause Polymerase Chain Reaction law.invention Geographical Locations 0302 clinical medicine law Genotype Medicine and Health Sciences 030212 general & internal medicine Child Escherichia coli Infections Polymerase chain reaction Multidisciplinary Antimicrobials Drugs Antimicrobial Anti-Bacterial Agents Denaturation Diarrhea Tetracyclines Medicine Female medicine.symptom Research Article medicine.drug_class Science 030106 microbiology Nigeria Gastroenterology and Hepatology Biology Research and Analysis Methods Microbiology beta-Lactamases 03 medical and health sciences Signs and Symptoms Antibiotic resistance Microbial Control Drug Resistance Bacterial Escherichia coli medicine Humans Molecular Biology Techniques Molecular Biology Pharmacology Biology and Life Sciences biochemical phenomena metabolism and nutrition bacterial infections and mycoses Genes Bacterial Antibiotic Resistance People and Places Africa Antimicrobial Resistance Clinical Medicine |
| Zdroj: | PLoS ONE PLoS ONE, Vol 15, Iss 12, p e0243130 (2020) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0243130 |
| Popis: | The increase in antimicrobial resistance in developed and developing countries is a global public health challenge. In this context β-lactamase production is a major contributing factor to resistance globally. The aim of this study was to determine the prevalence of phenotypic and genotypic extended spectrum β-lactamases (ESBLs) in 296 E. coli isolates recovered from diarrhoeic children younger than five years in Kano whose susceptibility profile against 7 antimicrobials had been determined. The E. coli isolates were subjected to double disc synergy test for phenotypic ESBLs detection and ESBL associated genes (blaCTX-M, blaTEM and blaSHV) were detected using conventional PCR. Phenotypically, 12.8% (38/296) E. coli isolates presented a ESBLs phenotype, with a significantly higher proportion in isolates from females compared with males (P-value = 0.024). blaCTX-M 73.3% and blaTEM 73.3% were the predominant resistance genes in the ESBLs positive E. coli (each detected in 22/30 isolates, of which 14 harboured both). In addition, 1/30 harboured blaCTX-M + blaTEM + blaSHV genes simultaneously. This study demonstrates the presence of ESBLs E. coli isolates in clinically affected children in Kano, and demonstrates the circulation of blaCTX-M and blaTEM associated with those phenotypes. Enactment of laws on prudent antibiotic use is urgently needed in Kano. |
| Databáze: | OpenAIRE |
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