DNA extraction method for PCR in mycorrhizal fungi

Autor:	P.R. Mills, S. Sreenivasaprasad, Sellamuthu Manian
Rok vydání:	2001
Předmět:	fungi Fungal genetics Fungi Biology Amplicon Applied Microbiology and Biotechnology DNA extraction Molecular biology Polymerase Chain Reaction law.invention genomic DNA chemistry.chemical_compound Biochemistry chemistry law Low copy number DNA Fungal Polymerase chain reaction DNA Mycelium
Zdroj:	Letters in applied microbiology. 33(4)
ISSN:	0266-8254
Popis:	Aims: To develop a simple and rapid DNA extraction protocol for PCR in mycorrhizal fungi. Methods and Results: The protocol combines the application of rapid freezing and boiling cycles and passage of the extracts through DNA purification columns. PCR amplifiable DNA was obtained from a number of endo- and ecto-mycorrhizal fungi using minute quantities of spores and mycelium, respectively. Conclusions: DNA extracted following the method, was used to successfully amplify regions of interest from high as well as low copy number genes. The amplicons were suitable for further downstream applications such as sequencing and PCR-RFLPs. Significance and Impact of the Study: The protocol described is simple, short and facilitates rapid isolation of PCR amplifiable genomic DNA from a large number of fungal isolates in a single day. The method requires only minute quantities of starting material and is suitable for mycorrhizal fungi as well as a range of other fungi.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f14cfd39888f0bba6909ccb395fbbc0f https://pubmed.ncbi.nlm.nih.gov/11559406 Zobrazit plný text záznamu Plný text