Enhancement of Lipid Extraction from Marine Microalga,ScenedesmusAssociated with High-Pressure Homogenization Process
| Autor: | Ji Seon Kim, Yong-Chang Seo, Ga-Vin Kim, Woon-Yong Choi, Do-Hyung Kang, Sung-Ho Oh, Hyeon-Yong Lee, Choon-Geun Lee, Shin Young Lee, Chi-Ho Song, Seok-Cheol Cho |
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| Rok vydání: | 2012 |
| Předmět: |
Article Subject
Scanning electron microscope lcsh:Biotechnology Oceans and Seas Health Toxicology and Mutagenesis Liquid-Liquid Extraction Kinetics lcsh:Medicine Cell Fractionation Homogenization (chemistry) Chemical kinetics Lipid extraction lcsh:TP248.13-248.65 Pressure Genetics Molecular Biology Scenedesmus Chromatography biology Chemistry lcsh:R General Medicine Lipid Metabolism biology.organism_classification Lipids Solvent Biochemistry Biodiesel production Molecular Medicine Research Article Biotechnology |
| Zdroj: | Journal of Biomedicine and Biotechnology Journal of Biomedicine and Biotechnology, Vol 2012 (2012) |
| ISSN: | 1110-7251 1110-7243 |
| Popis: | Marine microalga,Scenedesmussp., which is known to be suitable for biodiesel production because of its high lipid content, was subjected to the conventional Folch method of lipid extraction combined with high-pressure homogenization pretreatment process at 1200 psi and 35°C. Algal lipid yield was about 24.9% through this process, whereas only 19.8% lipid can be obtained by following a conventional lipid extraction procedure using the solvent, chloroform : methanol (2 : 1, v/v). Present approach requires 30 min process time and a moderate working temperature of 35°C as compared to the conventional extraction method which usually requires >5 hrs and 65°C temperature. It was found that this combined extraction process followed second-order reaction kinetics, which means most of the cellular lipids were extracted during initial periods of extraction, mostly within 30 min. In contrast, during the conventional extraction process, the cellular lipids were slowly and continuously extracted for >5 hrs by following first-order kinetics. Confocal and scanning electron microscopy revealed altered texture of algal biomass pretreated with high-pressure homogenization. These results clearly demonstrate that the Folch method coupled with high-pressure homogenization pretreatment can easily destruct the rigid cell walls of microalgae and release the intact lipids, with minimized extraction time and temperature, both of which are essential for maintaining good quality of the lipids for biodiesel production. |
| Databáze: | OpenAIRE |
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