Comparative transcriptome analysis revealed key factors for differential cadmium transport and retention in roots of two contrasting peanut cultivars
| Autor: | Yue Li, Caifeng Liu, Yuanyuan Ma, Xueling Du, Xin Li, Rugang Yu, Gangrong Shi |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine lcsh:QH426-470 Arachis Genotype lcsh:Biotechnology Chromosomal translocation Biology 01 natural sciences Plant Roots Transcriptome 03 medical and health sciences Gene Expression Regulation Plant lcsh:TP248.13-248.65 Gene expression Genetics Gene Cell wall modification Cation Transport Proteins Gene Library cDNA library Sequence Analysis RNA Gene Expression Profiling food and beverages lcsh:Genetics 030104 developmental biology Peanut Gene Ontology Root RNA Plant ATP-Binding Cassette Transporters DNA microarray 010606 plant biology & botany Biotechnology Research Article Cadmium |
| Zdroj: | BMC Genomics BMC Genomics, Vol 19, Iss 1, Pp 1-16 (2018) |
| ISSN: | 1471-2164 |
| Popis: | Background Peanut is the world’s fourth largest oilseed crop that exhibits wide cultivar variations in cadmium (Cd) accumulation. To establish the mechanisms of Cd distribution and accumulation in peanut plants, eight cDNA libraries from the roots of two contrasting cultivars, Fenghua 1 (low-Cd cultivar) and Silihong (high-Cd cultivar), were constructed and sequenced by RNA-sequencing. The expression patterns of 16 candidate DEGs were validated by RT-qPCR analysis. Results A total of 75,634 genes including 71,349 known genes and 4484 novel genes were identified in eight cDNA libraries, among which 6798 genes were found to be Cd-responsive DEGs and/or DEGs between these two cultivars. Interestingly, 183 DEGs encoding ion transport related proteins and 260 DEGs encoding cell wall related proteins were identified. Among these DEGs, nine metal transporter genes (PDR1, ABCC4 and ABCC15, IRT1, ZIP1, ZIP11, YSL7, DTX43 and MTP4) and nine cell wall related genes (PEs, PGIPs, GTs, XYT12 CYP450s, LACs, 4CL2, C4H and CASP5) showed higher expression in Fenghua 1 than in Silihong. Conclusions Both the metal transporters and cell wall modification might be responsible for the difference in Cd accumulation and translocation between Fenghua 1 and Silihong. These findings would be useful for further functional analysis, and reveal the molecular mechanism responsible for genotype difference in Cd accumulation. Electronic supplementary material The online version of this article (10.1186/s12864-018-5304-7) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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