Mechanistic insights into the digestion of complex dietary fibre by the rumen microbiota using combinatorial high-resolution glycomics and transcriptomic analyses

Autor: Ajay Badhan, Kristin E. Low, Darryl R. Jones, Xiaohui Xing, Mohammad Raza Marami Milani, Rodrigo Ortega Polo, Leeann Klassen, Sivasankari Venketachalam, Michael G. Hahn, D. Wade Abbott, Tim A. McAllister
Rok vydání: 2022
Předmět:
AG
arabinogalactan
Nutrient utilization
GH
glycosyl hydrolase
Rumen microbiome
AB
arabinan
Biophysics
Glycome profiling
Biochemistry
AO
ammonium oxalate
CAZyme
carbohydrate active enzyme
PC
post-chlorite
Structural Biology
PL
polysaccharide lyase
Genetics
XG
xyloglucan
NDF
neutral detergent fibre
HX
heteroxylan
Differential gene expression
BS
barley straw
Dietary polysaccharides
ComputingMethodologies_COMPUTERGRAPHICS
AGP
arabinogalactan protein
CE
carbohydrate esterase
TTIR
total tract indigestible residue
food and beverages
ADF
acid detergent fibre
HG
homogalacturonan
ELISA
enzyme-linked immunosorbent assay
AX
arabinoxylan
DE
differentially expressed
AIR
alcohol insoluble residue
HPAEC-PAD
high performance anion exchange chromatography coupled with pulsed amperometric detection
Computer Science Applications
RG
rhamnogalacturonan
FID
flame ionization detection GC
gas chromatography
MS
mass spectrometry
Glycoside hydrolase
SC
sodium carbonate
CH
chlorite
Transcriptome
TP248.13-248.65
CAZymes
Linkage analysis
mAbs
monoclonal antibodies
Research Article
Biotechnology
Zdroj: Computational and Structural Biotechnology Journal
Computational and Structural Biotechnology Journal, Vol 20, Iss, Pp 148-164 (2022)
ISSN: 2001-0370
DOI: 10.1016/j.csbj.2021.12.009
Popis: Graphical abstract
There is a knowledge gap regarding the factors that impede the ruminal digestion of plant cell walls or if rumen microbiota possess the functional activities to overcome these constraints. Innovative experimental methods were adopted to provide a high-resolution understanding of plant cell wall chemistries, identify higher-order structures that resist microbial digestion, and determine how they interact with the functional activities of the rumen microbiota. We characterized the total tract indigestible residue (TTIR) from cattle fed a low-quality straw diet using two comparative glycomic approaches: ELISA-based glycome profiling and total cell wall glycosidic linkage analysis. We successfully detected numerous and diverse cell wall glycan epitopes in barley straw (BS) and TTIR and determined their relative abundance pre- and post-total tract digestion. Of these, xyloglucans and heteroxylans were of higher abundance in TTIR. To determine if the rumen microbiota can further saccharify the residual plant polysaccharides within TTIR, rumen microbiota from cattle fed a diet containing BS were incubated with BS and TTIR ex vivo in batch cultures. Transcripts coding for carbohydrate-active enzymes (CAZymes) were identified and characterized for their contribution to cell wall digestion based on glycomic analyses, comparative gene expression profiles, and associated CAZyme families. High-resolution phylogenetic fingerprinting of these sequences encoded CAZymes with activities predicted to cleave the primary linkages within heteroxylan and arabinan. This experimental platform provides unprecedented precision in the understanding of forage structure and digestibility, which can be extended to other feed-host systems and inform next-generation solutions to improve the performance of ruminants fed low-quality forages.
Databáze: OpenAIRE
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