A new sensitive microassay for the measurement of erythrocyte glycogen
| Autor: | J Farquharson, E.Cherry Jamieson, R. W. Logan, Gordon B. MacPhee |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
chemistry.chemical_classification
Erythrocytes Hot Temperature Chromatography Glycogen Microchemistry Biochemistry (medical) Clinical Biochemistry 1-Propanol General Medicine Biology Carbohydrate Polysaccharide Biochemistry Red blood cell chemistry.chemical_compound medicine.anatomical_structure chemistry medicine Humans Quantitative analysis (chemistry) Ethanol precipitation |
| Zdroj: | Clinica Chimica Acta. 187:89-93 |
| ISSN: | 0009-8981 |
| DOI: | 10.1016/0009-8981(90)90334-o |
| Popis: | Conventional ethanol precipitation of sub-microgram amounts of glycogen leads to low yields (less than 50%). Quantitative recoveries of 90% were attained, however, when the isolation temperature was raised to 50 degrees C and ethanol was replaced by the less polar propan-2-ol. This improvement enabled development of an erythrocyte assay for glycogen which was both sensitive (0.1 microgram glycogen) and required only 1 ml of whole blood. 26 paediatric specimens were analysed and a reference range of values from undetected to 78 micrograms glycogen/g haemoglobin (Hb) was obtained. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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