A TPA-caged precursor of (imino)coumarin for 'turn-on' fluorogenic detection of Cu+
| Autor: | Caroline Brommesson, Jiwen Hu, Hui Wang, Qiong Zhang, Kajsa Uvdal, Hong-Wen Gao, Xuanjun Zhang, Zhangjun Hu, Yupeng Tian, Meng Zhao |
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| Rok vydání: | 2016 |
| Předmět: |
Tris
Pyridines chemistry.chemical_element 010402 general chemistry Photochemistry 01 natural sciences Biochemistry Analytical Chemistry chemistry.chemical_compound Coumarins Environmental Chemistry heterocyclic compounds Solubility Spectroscopy Fluorescent Dyes Aqueous solution Molecular Structure 010405 organic chemistry Coumarin Fluorescence Copper 0104 chemical sciences chemistry Titration Amine gas treating Imines |
| Zdroj: | Analytica Chimica Acta. 933:189-195 |
| ISSN: | 0003-2670 |
| DOI: | 10.1016/j.aca.2016.05.031 |
| Popis: | We strategize to utilize the precursors of (imino)coumarin fluorophores to deliver novel reactive Cu(+) probes, where tris[(2-pyridyl)-methyl] amine (TPA) works as a reactive receptor towards Cu(+). To verify this strategy, CP1, a representative probe and relevant sensing behaviors towards Cu(+) are presented here. CP1 features good solubility and fast response for monitoring labile copper in aqueous solution and live cells. The sensing mechanism of CP1 is determined by HPLC titration and mass spectrometric analysis. The probe CP1 exhibits a 60-fold fluorescence enhancement and a detection limitation of 10.8 nM upon the detection of Cu(+). CP1 is further applied for imaging labile copper in live cells. This work provides a starting point for future development of Cu(+) probes, based on in situ formation of (imino)coumarin scaffolds, as well as their further investigations of copper signaling and biological events. |
| Databáze: | OpenAIRE |
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