Selenium protects against cadmium-induced kidney apoptosis in chickens by activating the PI3K/AKT/Bcl-2 signaling pathway

Autor: Shu-Fang Zheng, Rong-Kun Bao, Xin-Yue Wang
Rok vydání: 2017
Předmět:
0301 basic medicine
medicine.medical_specialty
Health
Toxicology and Mutagenesis
Nitric Oxide Synthase Type II
Apoptosis
010501 environmental sciences
Kidney
Nitric Oxide
01 natural sciences
Mitochondrial Membrane Transport Proteins
Nitric oxide
03 medical and health sciences
chemistry.chemical_compound
Phosphatidylinositol 3-Kinases
Selenium
Internal medicine
medicine
Environmental Chemistry
Animals
Protein kinase B
PI3K/AKT/mTOR pathway
0105 earth and related environmental sciences
biology
Cytochrome c
General Medicine
Pollution
Molecular biology
Nitric oxide synthase
030104 developmental biology
Endocrinology
Mitochondrial respiratory chain
chemistry
Gene Expression Regulation
Proto-Oncogene Proteins c-bcl-2
biology.protein
Kidney Diseases
Signal transduction
Chickens
Proto-Oncogene Proteins c-akt
Cadmium
Signal Transduction
Zdroj: Environmental science and pollution research international. 24(25)
ISSN: 1614-7499
Popis: Cadmium (Cd) is a toxic heavy metal that can induce apoptosis. Selenium (Se) is a necessary trace element and can antagonize the toxicity of many heavy metals, including Cd. PI3K/AKT/Bcl-2 is a key survival signaling pathway that regulates cellular defense system against oxidative injury as well as cell proliferation, survival, and apoptosis. The antagonistic effects of Se on Cd-induced toxicity have been reported. However, little is known about the effect of Se on Cd-induced apoptosis in chicken kidneys via the PI3K/AKT/Bcl-2 signaling pathway. In the present study, we fed chickens with Se, Cd, or both Se and Cd supplements, and after 90 days of treatment, we detected the related index. The results showed that the activity of inducible nitric oxide synthase (iNOS) and concentration of nitric oxide (NO) were increased; activities of the mitochondrial respiratory chain complexes (complexes I, II, and V) and ATPases (the Na+-K+-ATPase, the Mg2+-ATPase, and the Ca2+-ATPase) were decreased; expression of PI3K, AKT, and Bcl-2 were decreased; and expression of Bax, Bak, P53, Caspase-3, Caspase-9, and cytochrome c (Cyt c) were increased. Additionally, the results of the TUNEL assay showed that the number of apoptotic cells was increased in the Cd group. By contrast, there was a significant improvement of the correlation indicators and occurrence of apoptosis in the Se + Cd group compared to the Cd group. In conclusion, our results confirmed that Se had a positive effect on ameliorating Cd-induced apoptosis in chicken kidney tissue by activating the PI3K/AKT/Bcl-2 signaling pathway.
Databáze: OpenAIRE
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