Rapid improvement in the macrolactins production of Bacillus sp. combining atmospheric room temperature plasma with the specific growth rate index

Autor:	Yonghong Liu, Yuman Gan, Lian Yu, Lei Jiang, Cheng-Hai Gao, Xiangxi Yi
Rok vydání:	2019
Předmět:	chemistry.chemical_classification Specific growth Strain (chemistry) Plasma Gases Mutant Fatty Acids Colony morphology Fatty acid Bioengineering Bacillus Bacillus sp Applied Microbiology and Biotechnology Indole Alkaloids Kinetics chemistry RNA Sequence Mutation Food science Biotechnology
Zdroj:	Journal of bioscience and bioengineering. 130(1)
ISSN:	1347-4421
Popis:	Macrolactins (MLNs) have attracted considerable attention due to their antibacterial and antiviral properties. Here, the MLN production of Bacillus sp. strain IMDGX0108 was improved using a breeding strategy of atmospheric room temperature plasma (ARTP) technique. Combining with a selection procedure based on the colony morphology and specific growth rate index (SGRI), two genetically stable mutants A29 and A72 were identified. The MLN production of A29 and A72 was 35.2% and 52.8% greater than that of the parent strain, respectively. The best-performing mutant A72 was subjected to RNA-sequence analysis. Five pathways were significantly enriched, and fatty acid bioprocesses might play an important role in improving the production of MLNs. The combined strategy developed herein (i.e., ARTP mutation plus an efficient screening procedure) might be an appropriate method by which to obtain strains overproducing MLNs.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f1391d6c3b6a87a38c233af3ddc0a0cb https://pubmed.ncbi.nlm.nih.gov/32224010 Zobrazit plný text záznamu Full Text from ScienceDirect