Anti-Neuroinflammatory and Anti-Inflammatory Activities of Phenylheptatriyne Isolated from the Flowers of Coreopsis lanceolata L. via NF-κB Inhibition and HO-1 Expression in BV2 and RAW264.7 Cells
Autor: | Linsha Dong, Zhiming Liu, Hwan Lee, Wonmin Ko, Dong-Sung Lee, Eun-Rhan Woo, Chi-Su Yoon |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Lipopolysaccharides
Anti-Inflammatory Agents Nitric Oxide Synthase Type II medicine.disease_cause Coreopsis lanceolata L chemistry.chemical_compound Mice 0302 clinical medicine 030212 general & internal medicine Biology (General) Spectroscopy 0303 health sciences biology Interleukin General Medicine mouse macrophage RAW 264.7 Computer Science Applications flower Chemistry Coreopsis lanceolata Tumor necrosis factor alpha Microglia Coreopsis Signal Transduction medicine.drug_class QH301-705.5 Flowers Nitric Oxide Catalysis Anti-inflammatory Article Dinoprostone Nitric oxide Proinflammatory cytokine Inorganic Chemistry 03 medical and health sciences medicine Animals Physical and Theoretical Chemistry Molecular Biology QD1-999 030304 developmental biology Inflammation Plant Extracts Macrophages Organic Chemistry biology.organism_classification Molecular biology anti-inflammation Leptosidin RAW 264.7 Cells chemistry Oxidative stress Heme Oxygenase-1 mouse microglia BV2 |
Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 7482, p 7482 (2021) International Journal of Molecular Sciences Volume 22 Issue 14 |
ISSN: | 1661-6596 1422-0067 |
Popis: | Aging is associated with immune disregulation and oxidative stress which lead to inflammation and neurodegenerative diseases. We have tried to identify the anti-neuroinflammatory and anti-inflammatory components of Coreopsis lanceolata L. The dried flowers of C. lanceolata were extracted with 70% EtOH, and the obtained extract was divided into CH2Cl2, EtOAc, n-BuOH, and H2O fractions. The CH2Cl2 fraction was separated using silica gel and C-18 column chromatography to yield phenylheptatriyne (1), 2′-hydroxy-3,4,4′-trimethoxychalcone (2), and 4′,7-dimethoxyflavanone (3). Additionally, the EtOAc fraction was subjected to silica gel, C-18, and Sephadex LH-20 column chromatography to yield 8-methoxybutin (4) and leptosidin (5). All the compounds isolated from C. lanceolata inhibited the production of nitric oxide (NO) in LPS-induced BV2 and RAW264.7 cells. In addition, phenylheptatriyne and 4′,7-dimethoxyflavanone reduced the secretion of inflammatory cytokines, tumor necrosis factor alpha (TNF-α), and interleukin (IL)-6. Among them, phenylheptatriyne was significantly downregulated in the expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2). Subsequently, phenylheptatriyne also effectively inhibited nuclear factor-kappa B (NF-κB) activation in LPS-stimulated BV2 and RAW264.7 cells. Based on these results, the anti-neuroinflammatory effect of phenylheptatriyne isolated from C. lanceolata was confirmed, which may exert a therapeutic effect in treatment of neuroinflammation-related diseases. |
Databáze: | OpenAIRE |
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