Anti-Neuroinflammatory and Anti-Inflammatory Activities of Phenylheptatriyne Isolated from the Flowers of Coreopsis lanceolata L. via NF-κB Inhibition and HO-1 Expression in BV2 and RAW264.7 Cells

Autor: Linsha Dong, Zhiming Liu, Hwan Lee, Wonmin Ko, Dong-Sung Lee, Eun-Rhan Woo, Chi-Su Yoon
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Lipopolysaccharides
Anti-Inflammatory Agents
Nitric Oxide Synthase Type II
medicine.disease_cause
Coreopsis lanceolata L
chemistry.chemical_compound
Mice
0302 clinical medicine
030212 general & internal medicine
Biology (General)
Spectroscopy
0303 health sciences
biology
Interleukin
General Medicine
mouse macrophage RAW 264.7
Computer Science Applications
flower
Chemistry
Coreopsis lanceolata
Tumor necrosis factor alpha
Microglia
Coreopsis
Signal Transduction
medicine.drug_class
QH301-705.5
Flowers
Nitric Oxide
Catalysis
Anti-inflammatory
Article
Dinoprostone
Nitric oxide
Proinflammatory cytokine
Inorganic Chemistry
03 medical and health sciences
medicine
Animals
Physical and Theoretical Chemistry
Molecular Biology
QD1-999
030304 developmental biology
Inflammation
Plant Extracts
Macrophages
Organic Chemistry
biology.organism_classification
Molecular biology
anti-inflammation
Leptosidin
RAW 264.7 Cells
chemistry
Oxidative stress
Heme Oxygenase-1
mouse microglia BV2
Zdroj: International Journal of Molecular Sciences, Vol 22, Iss 7482, p 7482 (2021)
International Journal of Molecular Sciences
Volume 22
Issue 14
ISSN: 1661-6596
1422-0067
Popis: Aging is associated with immune disregulation and oxidative stress which lead to inflammation and neurodegenerative diseases. We have tried to identify the anti-neuroinflammatory and anti-inflammatory components of Coreopsis lanceolata L. The dried flowers of C. lanceolata were extracted with 70% EtOH, and the obtained extract was divided into CH2Cl2, EtOAc, n-BuOH, and H2O fractions. The CH2Cl2 fraction was separated using silica gel and C-18 column chromatography to yield phenylheptatriyne (1), 2′-hydroxy-3,4,4′-trimethoxychalcone (2), and 4′,7-dimethoxyflavanone (3). Additionally, the EtOAc fraction was subjected to silica gel, C-18, and Sephadex LH-20 column chromatography to yield 8-methoxybutin (4) and leptosidin (5). All the compounds isolated from C. lanceolata inhibited the production of nitric oxide (NO) in LPS-induced BV2 and RAW264.7 cells. In addition, phenylheptatriyne and 4′,7-dimethoxyflavanone reduced the secretion of inflammatory cytokines, tumor necrosis factor alpha (TNF-α), and interleukin (IL)-6. Among them, phenylheptatriyne was significantly downregulated in the expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2). Subsequently, phenylheptatriyne also effectively inhibited nuclear factor-kappa B (NF-κB) activation in LPS-stimulated BV2 and RAW264.7 cells. Based on these results, the anti-neuroinflammatory effect of phenylheptatriyne isolated from C. lanceolata was confirmed, which may exert a therapeutic effect in treatment of neuroinflammation-related diseases.
Databáze: OpenAIRE
Externí odkaz: