Skin-bacteria communication: Involvement of the neurohormone Calcitonin Gene Related Peptide (CGRP) in the regulation of Staphylococcus epidermidis virulence
| Autor: | Takfarinas Kentache, Cécile Duclairoir Poc, Marc G. J. Feuilloley, Awa N’Diaye, Olivier Lesouhaitier, Camille Leclerc, Yoan Konto-Ghiorghi, Sylvie Chevalier, Julie Hardouin |
|---|---|
| Přispěvatelé: | Laboratoire de Microbiologie Signaux et Microenvironnement (LMSM), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Polymères Biopolymères Surfaces (PBS), Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut de Chimie du CNRS (INC)-Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Institut de Chimie du CNRS (INC)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Keratinocytes Staphylococcus aureus media_common.quotation_subject Calcitonin Gene-Related Peptide Virulence Substance P Gadolinium Calcitonin gene-related peptide medicine.disease_cause Article Microbiology 03 medical and health sciences chemistry.chemical_compound Staphylococcus epidermidis Cathelicidins medicine Humans Secretion Interleukin 8 Internalization ComputingMilieux_MISCELLANEOUS media_common Skin Nerve Endings Multidisciplinary biology integumentary system Chemistry Interleukin-8 Neuropeptides Staphylococcal Infections biology.organism_classification 030104 developmental biology [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Biofilms Antimicrobial Cationic Peptides |
| Zdroj: | Scientific Reports Scientific Reports, Nature Publishing Group, 2016, 6 (1), ⟨10.1038/srep35379⟩ |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep35379 |
| Popis: | Staphylococci can sense Substance P (SP) in skin, but this molecule is generally released by nerve terminals along with another neuropeptide, Calcitonin Gene Related Peptide (CGRP). In this study, we investigated the effects of αCGRP on Staphylococci. CGRP induced a strong stimulation of Staphylococcus epidermidis virulence with a low threshold (−12 M) whereas Staphylococcus aureus was insensitive to CGRP. We observed that CGRP-treated S. epidermidis induced interleukin 8 release by keratinocytes. This effect was associated with an increase in cathelicidin LL37 secretion. S. epidermidis displayed no change in virulence factors secretion but showed marked differences in surface properties. After exposure to CGRP, the adherence of S. epidermidis to keratinocytes increased, whereas its internalization and biofilm formation activity were reduced. These effects were correlated with an increase in surface hydrophobicity. The DnaK chaperone was identified as the S. epidermidis CGRP-binding protein. We further showed that the effects of CGRP were blocked by gadolinium chloride (GdCl3), an inhibitor of MscL mechanosensitive channels. In addition, GdCl3 inhibited the membrane translocation of EfTu, the Substance P sensor. This work reveals that through interaction with specific sensors S. epidermidis integrates different skin signals and consequently adapts its virulence. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|