The Douglas-fir BiP promoter is functional in Arabidopsis and responds to wounding
| Autor: | Santosh Misra, Benjamin S. Forward, Milan Osusky |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Recombinant Fusion Proteins
Transgene Molecular Sequence Data Arabidopsis Germination GUS reporter system Plant Science Biology Gene Expression Regulation Plant Gene expression Genetics Arabidopsis thaliana Cloning Molecular Promoter Regions Genetic Glucuronidase Plant Proteins Reporter gene Base Sequence Arabidopsis Proteins Inverse polymerase chain reaction food and beverages Promoter Plants Genetically Modified biology.organism_classification Pseudotsuga Cell biology Seeds Stress Mechanical Carrier Proteins |
| Zdroj: | Planta. 215:569-576 |
| ISSN: | 1432-2048 0032-0935 |
| Popis: | A DNA sequence representing the promoter region of the Douglas-fir (Pseudotsuga menziesii [Mirb.] Franco) luminal binding protein PmBiP (PmBiPPro1) was isolated using inverse polymerase chain reaction (iPCR). Transient expression analysis of PmBiPPro1 fused to the beta-glucuronidase (GUS) reporter gene demonstrated that this promoter is functional in germinating Douglas-fir embryos. Transgenic Arabidopsis plants containing PmBiPPro1:GUS reporter gene constructs revealed strong staining associated with actively dividing/expanding cells and secretory tissues in developing seedlings. Wounding of cotyledons resulted in an increase in local staining associated with cells surrounding the wound site. Deletion analysis showed that elements necessary for basal-level expression reside within a -261 to +16 bp region, although upstream elements are necessary for higher-level expression in germinating Douglas-fir embryos, developing Arabidopsis seedlings and wounded cotyledons. Correlation of the observed expression pattern with the known function of BiP suggests that pathways controlling expression are highly conserved between angiosperms and gymnosperms. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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