Therapy with resveratrol attenuates obesity-associated allergic airway inflammation in mice
| Autor: | Diana Majolli André, Luiz O. Leiria, Natalia Tobar, Carolina Sollon, Edson Antunes, Marina C. Calixto, Gabriel Forato Anhê, Eduardo C. Alexandre |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Immunology Mice Inbred Strains Inflammation AMP-Activated Protein Kinases Resveratrol medicine.disease_cause Antioxidants Mice 03 medical and health sciences chemistry.chemical_compound Insulin resistance AMP-activated protein kinase Cell Movement Internal medicine Stilbenes medicine Animals Immunology and Allergy Obesity Lung Cells Cultured Pharmacology biology medicine.diagnostic_test AMPK Pneumonia medicine.disease Asthma Eosinophils Nitric oxide synthase 030104 developmental biology Bronchoalveolar lavage Endocrinology chemistry Disease Progression biology.protein medicine.symptom Oxidative stress |
| Zdroj: | International Immunopharmacology. 38:298-305 |
| ISSN: | 1567-5769 |
| DOI: | 10.1016/j.intimp.2016.06.017 |
| Popis: | Obesity and insulin resistance have been associated with deterioration in asthma outcomes. High oxidative stress and deficient activation of AMP-activated protein kinase (AMPK) have emerged as important regulators linking insulin resistance and inflammation. This study aimed to evaluate the effects of resveratrol on obesity-associated allergic pulmonary inflammation. Male C57/Bl6 mice fed with high-fat diet to induce obesity (obese group) or standard-chow diet (lean group) were treated or not with resveratrol (100mg/kg/day, two weeks). Mice were sensitized and challenged with ovalbumin (OVA). At 48h thereafter, bronchoalveolar lavage fluid was performed, and lungs collected for morphological studies and Western blot analysis. Treatment of obese mice with resveratrol significantly reduced hyperglycemia and insulin resistance, as well as the body measures (body mass, fat mass, % fat, and body area). OVA-challenge promoted a higher increase in pulmonary eosinophil infiltration in obese compared with lean mice, which was nearly abrogated by resveratrol treatment. Resveratrol markedly increased the phosphorylated AMPK expression in lung tissues of obese compared with lean mice. Resveratrol reduced the p47phox expression and reactive-oxygen species (ROS) production, and elevated the superoxide dismutase (SOD) levels in lung tissues of obese mice. The increased pulmonary levels of TNF-α and inducible nitric oxide synthase (iNOS) in obese mice were also normalized after resveratrol treatment. In lean mice, resveratrol failed to affect the levels of fasting glucose, p47phox, ROS levels, TNF-α, iNOS and phosphorylated AMPK. Resveratrol exhibits protective effects in obesity-associated lung inflammation that is accompanied by local AMPK activation and antioxidant property. |
| Databáze: | OpenAIRE |
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