Functional assay for shiga-like toxin via detection by antibody capture and multivalent galabiose binding
| Autor: | Jos P. M. van Putten, Hilde van Hattum, Kim van der Zwaluw, Gerben M. Visser, Roland J. Pieters, Rob Ruijtenbeek |
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| Rok vydání: | 2012 |
| Předmět: |
Functional assay
Clinical Biochemistry Dose dependence Pharmaceutical Science Biotin medicine.disease_cause Disaccharides Ligands Shiga Toxins Biochemistry Antibodies Antigen-Antibody Reactions chemistry.chemical_compound Shiga-like toxin Drug Discovery medicine Escherichia coli Molecular Biology Antigens Bacterial Binding Sites Capture antibody Toxin Chemistry Functional protein Organic Chemistry Serum Albumin Bovine Ligand (biochemistry) Molecular biology Molecular Medicine |
| Zdroj: | Bioorganicmedicinal chemistry letters. 22(24) |
| ISSN: | 1464-3405 |
| Popis: | A functional detection assay was developed for Escherichia coli secreted shiga-like toxin based on antibody capture and visualization with a multivalent galabiose ligand. It was possible to detect verotoxin in medically relevant E. coli samples in a dose dependent fashion. This method is a new step towards measuring functional protein levels in complex mixtures, which can be used for diagnostic purposes in a clinical setting. |
| Databáze: | OpenAIRE |
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