Gas chromatography-mass spectrometry determination of matrine in human plasma
| Autor: | Francis C. P. Law, Dan S T Sit, Guanghua Gao, Paul C. H. Li |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Detection limit
Chromatography Clinical Biochemistry Extraction (chemistry) Reproducibility of Results Cell Biology General Medicine Reference Standards Mass spectrometry Biochemistry Gas Chromatography-Mass Spectrometry Analytical Chemistry chemistry.chemical_compound Alkaloids Matrine chemistry Liquid–liquid extraction Humans Gas chromatography Gas chromatography–mass spectrometry Matrines Quantitative analysis (chemistry) Quinolizines |
| Zdroj: | Journal of Chromatography B. 808:209-214 |
| ISSN: | 1570-0232 |
| Popis: | A method was developed for the quantification of matrine in human plasma using a liquid-liquid extraction procedure followed by gas-chromatography-mass spectrometry (GC/MS) analysis. Deuterated matrine, an internal standard of the analysis, was spiked into the plasma samples before extraction. Linear detection responses were obtained for matrine concentrations ranging from 10 to 500 ng/ml. The intra-day and inter-day precision ranged from 0.4 to 4.0% and 1.0-3.5%, respectively. The intra-day accuracy was between -7.3 and 4.5%. The limit of quantification for matrine was 23 ng/ml. The extraction efficiency averaged about 38%. The validated GC/MS method will be used to quantify matrine in human plasma samples collected in a clinical trial study. |
| Databáze: | OpenAIRE |
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