Osteoclasts generate cross-linked collagen N-telopeptides (NTx) but not free pyridinolines when cultured on human bone

Autor: M.Y. Lee, David R. Eyre, S. Apone
Rok vydání: 1997
Předmět:
Hydrocortisone
Bone disease
Physiology
Endocrinology
Diabetes and Metabolism
Anti-Inflammatory Agents
Osteoclasts
Mice
chemistry.chemical_compound
Bone Marrow
Femur
Amino Acids
Cells
Cultured
Chromatography
High Pressure Liquid
Tartrate-resistant acid phosphatase
Mice
Inbred BALB C
Pyridinoline
Alendronate
Resorption
Isoenzymes
medicine.anatomical_structure
Biochemistry
Female
Collagen
Type I collagen
Calcitonin
Peptide Biosynthesis
medicine.medical_specialty
Histology
Acid Phosphatase
Enzyme-Linked Immunosorbent Assay
Biology
Collagen Type I
Bone resorption
Calcitriol
Osteoclast
Internal medicine
medicine
Animals
Humans
Bone Resorption
Dose-Response Relationship
Drug
Tartrate-Resistant Acid Phosphatase
Receptors
Calcitonin
medicine.disease
Culture Media
Endocrinology
chemistry
Dentin
Peptides
human activities
Biomarkers
Zdroj: Bone. 21:129-136
ISSN: 8756-3282
DOI: 10.1016/s8756-3282(97)00105-1
Popis: Urinary excretion of the cross-linked alpha 2(I) N-telopeptide (NTx) of type I collagen has proven in clinical studies to provide a highly responsive and specific index of bone resorption. In order to understand better the biological basis of the specificity and responsiveness of this marker, we examined whether osteoclasts cultured on human bone could generate immunoreactive NTx peptide. Mouse bone marrow cultures stimulated with 1,25 diliydroxyvitamin D3 (1,25(OH)2D3) and hydrocortisone to produce osteoclasts, were cocultured on human bone particles or dentin slices. Aliquots of culture medium were assayed for NTx by enzyme-linked immunosorbent assay (ELISA). NTx was detected in the medium 5 days after the addition of bone and continued to be produced linearly over the 14-day culture period. NTx production required attachment to the bone particles or dentin slices of mononuclear and multinuclear cells that stained for tartrate-resistant acid phosphatase. Surface area of resorbed dentin was highly correlated with medium NTx concentration (R2 = 0.84). Production of NTx was suppressed by the osteoclast inhibitors, calcitonin and alendronate, in a dose-dependent manner. Two other markers of bone resorption, hydroxylysyl pyridinoline and lysyl pyridinoline, were found in peptide linkage in the culture medium but not in free form; indicating that the osteoclasts had degraded the bone collagen to peptides but not to the free cross-linking amino acids.
Databáze: OpenAIRE
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