Isolation of lipase producer and its performance in enantioselective hydrolysis of glycidyl butyrate
| Autor: | Shi-Ying Jia, Jian-He Xu, Qing Shan Li, Jun Tang Yu |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Triacylglycerol lipase
Bioengineering Butyrate Applied Microbiology and Biotechnology Biochemistry Sensitivity and Specificity Hydrolysis Rhizopus Species Specificity Enzyme Stability Organic chemistry Lipase Molecular Biology Soil Microbiology biology Mycelium Chemistry Enantioselective synthesis Temperature Reproducibility of Results Stereoisomerism General Medicine Hydrogen-Ion Concentration biology.organism_classification Enzyme Activation Butyrates Biocatalysis biology.protein Enantiomer Biotechnology |
| Zdroj: | Applied biochemistry and biotechnology. 104(1) |
| ISSN: | 0273-2289 |
| Popis: | A racemic glycidyl butyrate resolving strain, preliminarily identified as a Rhizopus sp., had been isolated from soil. Its extracellular lipase was found to enantioselectively hydrolyze the (S)-enantiomer of the chiral ester, with optimal activities at pH 5.3 and 42 degrees C. Higher enantioselectivity of the enzyme was observed at lower temperatures, while the best enantioselectivity was obtained at pH 5.5-6.0, with an E value (enantiomeric ratio) of 57. |
| Databáze: | OpenAIRE |
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