| Autor: |
Modrow, Susanne, Höflacher, B., Mertz, R., Wolf, Hans J. |
| Jazyk: |
angličtina |
| Rok vydání: |
1989 |
| Předmět: |
|
| Popis: |
Chemically synthesize carrier-bound peptides have been used as antigens in diagnostic test systems (ELISA) and for raising antipeptide-specific antisera. The method does not require prior cleavage of the peptides from the support used for the solid-phase synthesis. Using the same resin for both the synthesis and the subsequent applications it was possible to avoid expensive and time-consuming purification procedures and artificial recoupling to solid supports. A quick and specific ELISA-based diagnostic test system for HIV-specific antipeptide antibodies in human sera was established. In addition the carrier-bound peptides were shown to be potent antigens for raising antibodies in animals. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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