Detection of human papillomavirus DNA in breast cancer of patients with cervical cancer history
| Autor: | Christian Marth, Annemarie Wiedemair, Elisabeth Mueller-Holzner, Thomas Brunhuber, Andreas Widschwendter |
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| Rok vydání: | 2004 |
| Předmět: |
Adult
CA15-3 Oncology medicine.medical_specialty Pathology Axillary lymph nodes Uterine Cervical Neoplasms Breast Neoplasms Cervical intraepithelial neoplasia medicine.disease_cause Breast cancer Virology Internal medicine medicine Humans Papillomaviridae skin and connective tissue diseases Aged Cervical cancer biology business.industry Papillomavirus Infections virus diseases Cancer Middle Aged Uterine Cervical Dysplasia medicine.disease biology.organism_classification female genital diseases and pregnancy complications Infectious Diseases medicine.anatomical_structure Immunoglobulin G DNA Viral Female Lymph Nodes business Carcinogenesis |
| Zdroj: | Journal of Clinical Virology. 31:292-297 |
| ISSN: | 1386-6532 |
| DOI: | 10.1016/j.jcv.2004.06.009 |
| Popis: | Background: Recent studies have revealed a possible role for the human papillomavirus (HPV) in the pathogenesis of breast cancer. In this study, patients having both a history of invasive cervical cancer and breast cancer as second primary cancer were selected for enrolment in a study of breast carcinomas for the presence of HPV. Methods: Paraffin-embedded tissue from cervical cancer, pelvic lymph nodes, breast cancer and axillary lymph nodes of eleven patients were examined for the presence of HPV DNA using a polymerase chain reaction – enzyme immuno assay. DNA extraction was performed with the “QIAamp Tissue Kit” according to the manufacturer’s instructions. Additionally, serum samples taken between diagnosis of cervical and breast cancer, were analyzed for the presence of HPV DNA to examine a possible haematogenous spread of oncogenic HPV DNA. Results: All cervical carcinomas were HPV-positive. HPV DNA was detected in seven out of eleven cases in breast cancer and/or axillary lymph node tissue. Six patients had the same HPV type (HPV-16) in cervical cancer and in the corresponding breast cancer/lymph node tissue. In one case, the same HPV DNA type (HPV 16) was detected in cervical cancer, breast cancer and serum sample. Conclusion: These results suggest that HPV DNA might be transported from the original site of infection to the breast tissue by the bloodstream, and that it is possibly involved in the carcinogenesis of breast neoplasia in some patients. |
| Databáze: | OpenAIRE |
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