The Effect of Uterine Lavage on Soluble CD14, Chemokine Ligand 2, and Interleukin 10 Levels in Mares With Postpartum Metritis
| Autor: | Elina Tukia, Bettina Wagner, Maria Kareskoski, Kirsi Vainio, Jenni Mönki |
|---|---|
| Přispěvatelé: | Production Animal Hospital, Production Animal Medicine, Equine and Small Animal Medicine, Equine Hospital, Animal Reproduction Science, Helsinki One Health (HOH) |
| Rok vydání: | 2021 |
| Předmět: |
Chemokine
040301 veterinary sciences Lipopolysaccharide Receptors Uterus Inflammation 413 Veterinary science Ligands 0403 veterinary science Andrology 03 medical and health sciences medicine Animals Horses Metritis Therapeutic Irrigation Retained fetal membranes 030304 developmental biology 0303 health sciences Leukopenia biology Equine business.industry Postpartum Period Interleukin-10 (IL-10) Interleukin Postpartum metritis 04 agricultural and veterinary sciences Laminitis Soluble CD14 sCD14) medicine.disease Interleukin-10 3. Good health Interleukin 10 medicine.anatomical_structure Chemokine ligand 2 (CCL2) biology.protein Female Chemokines medicine.symptom business |
| Zdroj: | Journal of Equine Veterinary Science. 98:103365 |
| ISSN: | 0737-0806 |
| Popis: | Postpartum metritis in mares is a life-threatening condition associated with severe clinical signs due to endotoxemia, and it is often followed by complications such as laminitis. Repeated large-volume uterine lavages are commonly recommended as a part of the treatment protocol to remove endotoxin-laden contents from the uterus. It has, however, also been suggested that lavages may increase the uptake of endotoxin into the circulation, leading to a deterioration of clinical signs. Endotoxemia is associated with the release of a multitude of inflammatory mediators regulating the immune response. The aim of this study was to evaluate if uterine lavage influences serum levels of the inflammation markers soluble CD14 (sCD14), chemokine (C-C motif) ligand 2 (CCL2), and interleukin (IL)-10 in mares with postpartum metritis. Serum samples were collected from eight mares treated for metritis at a university teaching hospital. Mares with fever, tachycardia, and/or leukopenia and hemosanguineous or purulent intrauterine fluid within 1 week of foaling were included in the study. Serum samples were taken before uterine lavage and 15 and 30 minutes after starting the lavage. The concentrations of sCD14, CCL2, and IL 10 were determined with a fluorescent beadebased immunoassay. There were no significant differences between sCD14, CCL2, or IL-10 levels at different sampling times. Heart rate was significantly lower after uterine lavage than before. The differences in body temperature and leukocyte count before and after lavage were not significant. In conclusion, uterine lavage had no immediate effect on the serum concentration of sCD14, CCL2, or IL-10, nor did it affect clinical parameters negatively. (c) 2020 Elsevier Inc. All rights reserved. |
| Databáze: | OpenAIRE |
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