A Micromethod for Lymphoblastic Transformation of Mouse Lymphocytes from Peripheral Blood
| Autor: | H. Meier, H. J. Heiniger, H W Chen, J. M. Wolf |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Cell
Heterologous Biology Lymphocyte Activation Tritium General Biochemistry Genetics and Molecular Biology Mice Mice Inbred AKR Micromanipulation Tissue culture Culture Techniques Lectins Methods medicine Animals Bioassay Cells Cultured Cell growth Molecular biology Peripheral blood Culture Media Peripheral Mice Inbred C57BL Transformation (genetics) medicine.anatomical_structure Immunology |
| Zdroj: | Experimental Biology and Medicine. 143:6-11 |
| ISSN: | 1535-3699 1535-3702 |
| DOI: | 10.3181/00379727-143-37242 |
| Popis: | Although several micromethods for culturing human peripheral lymphocytes exist (1–3), attempts to develop similar procedures for small laboratory animals have not been equally successful (4–6). First, mouse lymphocytes appear less responsive to PHA stimulation than human lymphocytes under their respective culture conditions (7, 8). Second, because cell suspensions from whole organs have generally been used from mice, direct comparisons to human peripheral lymphocytes are difficult; also, the mice had to be killed, thus eliminating them from further use or breeding experiments. Third, the culture systems for mouse lymphocytes employed varying amounts of heterologous serum (5, 6) or lacked appropriate standardization with respect to optimal culturing conditions and culture time. The lack of a fast and reproducible micromethod for lympho-blastogenic transformation in mice is regrettable because it precludes studies on the genetic control of transformation and initiation of cell proliferation without sacrific... |
| Databáze: | OpenAIRE |
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