Effect of Interferon-γ on the Basal and the TNFα-Stimulated Secretion of CXCL8 in Thyroid Cancer Cell Lines Bearing Either the RET/PTC Rearrangement Or the BRAF V600e Mutation

Autor: Luca Chiovato, Vittorio Abbonante, Christian A. Di Buduo, Patrizia Pignatti, Mario Rotondi, Alessandra Balduini, Flavia Magri, Francesca Coperchini, Oriana Awwad
Rok vydání: 2016
Předmět:
0301 basic medicine
musculoskeletal diseases
Proto-Oncogene Proteins B-raf
medicine.medical_specialty
Article Subject
Immunology
Cell
Enzyme-Linked Immunosorbent Assay
Biology
03 medical and health sciences
Interferon-gamma
0302 clinical medicine
Cell Movement
Internal medicine
Cell Line
Tumor
lcsh:Pathology
medicine
Humans
Interferon gamma
Secretion
Interleukin 8
Thyroid Neoplasms
RET/PTC Rearrangement
Gene Rearrangement
Wound Healing
Tumor Necrosis Factor-alpha
Interleukin-8
Proto-Oncogene Proteins c-ret
Cell migration
Cell Biology
Gene rearrangement
Molecular biology
Chemokine CXCL10
Gene Expression Regulation
Neoplastic
030104 developmental biology
Endocrinology
medicine.anatomical_structure
Cell culture
030220 oncology & carcinogenesis
Mutation
lcsh:RB1-214
medicine.drug
Research Article
Zdroj: Mediators of Inflammation
Mediators of Inflammation, Vol 2016 (2016)
ISSN: 1466-1861
Popis: CXCL8 displays several tumor-promoting effects. Targeting and/or lowering CXCL8 concentrations within the tumor microenvironment would produce a therapeutic benefit. Aim of this study was to test the effect of IFNγon the basal and TNFα-stimulated secretion of CXCL8 in TCP-1 and BCPAP thyroid cancer cell lines (harboring RET/PTC rearrangement and BRAF V600e mutation, resp.). Cells were incubated with IFNγ(1, 10, 100, and 1000 U/mL) alone or in combination with TNF-α(10 ng/mL) for 24 hours. CXCL8 and CXCL10 concentrations were measured in the cell supernatants. IFNγinhibited in a dose-dependent and significant manner both the basal (ANOVAF: 22.759;p<0.00001) and the TNFα-stimulated (ANOVAF: 15.309;p<0.00001) CXCL8 secretions in BCPAP but not in TPC-1 cells (NS). On the other hand, IFNγand IFNγ+ TNF-αinduced a significant secretion of CXCL10 in both BCPAP (p<0.05) and TPC-1 (p<0.05) cells. Transwell migration assay showed that (i) CXCL8 increased cell migration in both TPC-1 and BCPAP cells; (ii) IFNγsignificantly reduced the migration only of BCPAP cells; and (iii) CXCL8 reverted the effect of IFNγ. These results constitute the first demonstration that IFNγinhibits CXCL8 secretion and in turn the migration of a BRAF V600e mutated thyroid cell line.
Databáze: OpenAIRE
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