Evaluating the Effect of Sarconesiopsis magellanica (Diptera: Calliphoridae) Larvae-Derived Haemolymph and Fat Body Extracts on Chronic Wounds in Diabetic Rabbits

Autor: Alejandro Ramírez-Hernández, Jennifher Góngora, Andrea Díaz-Roa, María Antonia Gaona, Jesús A. Cortés-Vecino, Felio J. Bello, Manuel A. Patarroyo
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Male
Staphylococcus aureus
animal structures
Article Subject
Endocrinology
Diabetes and Metabolism

Fat Body
Physiology
nitrofural
medicine.disease_cause
lcsh:Diseases of the endocrine glands. Clinical endocrinology
Lucilia
alloxan
Diabetes Mellitus
Experimental

Microbiology
chemistry.chemical_compound
Endocrinology
Sarconesiopsis magellanica fat body extract
Hemolymph
Alloxan
medicine
Animals
Pseudomonas Infections
Calliphoridae
Nitrofural
Cell Proliferation
Wound Healing
lcsh:RC648-665
biology
Diptera
Diabetes
fungi
wound healing promoting agent
alloxan-induced diabetes mellitus
Staphylococcal Infections
biology.organism_classification
unclassified drug
Disease Models
Animal

chemistry
Larva
Pseudomonas aeruginosa
Instar
Heridas
Rabbits
Wound healing
Incidencia & prevención de la enfermedad
Research Article
medicine.drug
Zdroj: Journal of Diabetes Research
Repositorio EdocUR-U. Rosario
Universidad del Rosario
instacron:Universidad del Rosario
Journal of Diabetes Research, Vol 2015 (2015)
ISSN: 2314-6745
DOI: 10.1155/2015/270253
Popis: We evaluated extracts taken fromS. magellanicathird instar larvae fat body and haemolymph using a diabetic rabbit model and compared this to the effect obtained with the same substances taken fromLucilia sericatalarvae. Alloxan (a toxic glucose analogue) was used to induce experimental diabetes in twelve rabbits. Dorsal wounds were made in each animal and they were infected withStaphylococcus aureusandPseudomonas aeruginosa. They were then treated with haemolymph and lyophilized extracts taken from the selected blowflies’ larvae fat bodies. Each wound was then evaluated by using rating scales and histological analysis. More favourable scores were recorded on the PUSH and WBS scales for the wounds treated with fat body derived from the larvae of both species compared to that obtained with haemolymph; however, wounds treated with the substances taken fromS. magellanicahad better evolution. Histological analysis revealed that treatment led to tissue proliferation and more effective neovascularisation in less time with both species’ fat body extracts compared to treatment with just haemolymph. The results suggest the effectiveness of the substances evaluated and validate them in the animal model being used here as topical agents in treating chronic wounds.
Databáze: OpenAIRE