Evaluating the Effect of Sarconesiopsis magellanica (Diptera: Calliphoridae) Larvae-Derived Haemolymph and Fat Body Extracts on Chronic Wounds in Diabetic Rabbits
Autor: | Alejandro Ramírez-Hernández, Jennifher Góngora, Andrea Díaz-Roa, María Antonia Gaona, Jesús A. Cortés-Vecino, Felio J. Bello, Manuel A. Patarroyo |
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Jazyk: | angličtina |
Rok vydání: | 2015 |
Předmět: |
Male
Staphylococcus aureus animal structures Article Subject Endocrinology Diabetes and Metabolism Fat Body Physiology nitrofural medicine.disease_cause lcsh:Diseases of the endocrine glands. Clinical endocrinology Lucilia alloxan Diabetes Mellitus Experimental Microbiology chemistry.chemical_compound Endocrinology Sarconesiopsis magellanica fat body extract Hemolymph Alloxan medicine Animals Pseudomonas Infections Calliphoridae Nitrofural Cell Proliferation Wound Healing lcsh:RC648-665 biology Diptera Diabetes fungi wound healing promoting agent alloxan-induced diabetes mellitus Staphylococcal Infections biology.organism_classification unclassified drug Disease Models Animal chemistry Larva Pseudomonas aeruginosa Instar Heridas Rabbits Wound healing Incidencia & prevención de la enfermedad Research Article medicine.drug |
Zdroj: | Journal of Diabetes Research Repositorio EdocUR-U. Rosario Universidad del Rosario instacron:Universidad del Rosario Journal of Diabetes Research, Vol 2015 (2015) |
ISSN: | 2314-6745 |
DOI: | 10.1155/2015/270253 |
Popis: | We evaluated extracts taken fromS. magellanicathird instar larvae fat body and haemolymph using a diabetic rabbit model and compared this to the effect obtained with the same substances taken fromLucilia sericatalarvae. Alloxan (a toxic glucose analogue) was used to induce experimental diabetes in twelve rabbits. Dorsal wounds were made in each animal and they were infected withStaphylococcus aureusandPseudomonas aeruginosa. They were then treated with haemolymph and lyophilized extracts taken from the selected blowflies’ larvae fat bodies. Each wound was then evaluated by using rating scales and histological analysis. More favourable scores were recorded on the PUSH and WBS scales for the wounds treated with fat body derived from the larvae of both species compared to that obtained with haemolymph; however, wounds treated with the substances taken fromS. magellanicahad better evolution. Histological analysis revealed that treatment led to tissue proliferation and more effective neovascularisation in less time with both species’ fat body extracts compared to treatment with just haemolymph. The results suggest the effectiveness of the substances evaluated and validate them in the animal model being used here as topical agents in treating chronic wounds. |
Databáze: | OpenAIRE |
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