Smudge cells percentage on blood smear is a reliable prognostic marker in chronic lymphocytic leukemia
| Autor: | Moussa Seck, Awa Oumar Touré, Martine Raphael, Seynabou Fall, Abibatou Sall, Fatimata Bintou Sall, Fatou Samba Diago Ndiaye, Saliou Diop, Blaise Felix Faye, Macoura Gadji |
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| Rok vydání: | 2022 |
| Předmět: |
medicine.medical_specialty
Poor prognosis medicine.diagnostic_test business.industry Chronic lymphocytic leukemia Hematology 030204 cardiovascular system & hematology medicine.disease Gastroenterology Cytogenetic Aberrations Flow cytometry 03 medical and health sciences 0302 clinical medicine Blood smear Internal medicine medicine Immunology and Allergy business Prospective cohort study Smudge cells 030215 immunology Fluorescence in situ hybridization |
| Zdroj: | Hematology, Transfusion and Cell Therapy. 44:63-69 |
| ISSN: | 2531-1379 |
| DOI: | 10.1016/j.htct.2021.04.002 |
| Popis: | We evaluated the relevance of using the smudge cell percentage in the blood smear as a prognostic marker in CLL.In this prospective study, 42 untreated Senegalese patients with CLL were enrolled. The diagnosis was established, based on the peripheral blood count and flow cytometry using the Matutes score. Cytogenetic aberrations, assessed by fluorescence in situ hybridization (FISH), were available for 30 patients, while the immunoglobulin heavy chain genes (IGVH) mutation status was performed by next-generation sequencing (NGS) in 24 patients. The SC percentage was determined in the blood smear, as previously described. Statistical analyses were executed using the GraphPad Prism 8.The mean age was 63 years (48 - 85) and the male: female sex ratio was 4.66. A low SC (30%) percentage was correlated with Binet stage B/C (p = 0.0009), CD38 expression (p = 0.039), unmutated IGVH status (p = 0.0009) and presence of cytogenetic abnormalities (for del 13q, p = 0.0012, while for other cytogenetic aberrations, p = 0.016). An inverse correlation was found between the SC percentage and the absolute lymphocyte count (r = -0.51) and patients with higher percentage of SCs had a prolonged survival. However, there was no correlation between the SC percentage and age (p = 0.41) or gender (median, 19% for males vs. 20% for females; p = 0.76).When less than 30%, the SC was associated with a poor prognosis in CLL. Easy and affordable, the percentage of SCs in a blood smear could be a reliable prognostic marker, accessible to all CLL patients, mainly those in developing countries. |
| Databáze: | OpenAIRE |
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