The mRNA Distribution of Cancer Stem Cell Marker CD90/Thy-1 Is Comparable in Hepatocellular Carcinoma of Eastern and Western Populations
| Autor: | Thao P. T. Doan, Paola Tarchi, Caecilia H. C. Sukowati, Loraine Kay D. Cabral, An B. Luong, Deborah Bonazza, Huy Q. Do, Claudio Tiribelli, Hoa L. T. Pham, Lory Saveria Crocè, Long D. C. Tran, Cristina Bottin |
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| Přispěvatelé: | Luong, A. B., Do, H. Q., Tarchi, P., Bonazza, D., Bottin, C., Cabral, L. K. D., Tran, L. D. C., Doan, T. P. T., Croce, L. S., Pham, H. L. T., Tiribelli, C., Sukowati, C. H. C. |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
cancer stem cells
Male Pathology medicine.medical_treatment Messenger hepatocellular carcinoma mRNA expression Aged Biomarkers Tumor Carcinoma Hepatocellular Cohort Studies Female Gene Expression Regulation Neoplastic Hepatitis B Humans Liver Neoplasms Middle Aged Neoplastic Stem Cells Prognosis RNA Messenger Thy-1 Antigens 0302 clinical medicine Gene expression lcsh:QH301-705.5 Tumor medicine.diagnostic_test Incidence (epidemiology) General Medicine Liver Neoplasm 030220 oncology & carcinogenesis Hepatocellular carcinoma 030211 gastroenterology & hepatology Human medicine.medical_specialty cancer stem cell Prognosi Biology Immunofluorescence Article 03 medical and health sciences Cancer stem cell medicine CD90 Neoplastic Messenger RNA Carcinoma Hepatocellular medicine.disease digestive system diseases Gene Expression Regulation lcsh:Biology (General) RNA Neoplastic Stem Cell Hepatectomy Cohort Studie Biomarkers |
| Zdroj: | Cells Volume 9 Issue 12 Cells, Vol 9, Iss 2672, p 2672 (2020) |
| Popis: | Epidemiology of hepatocellular carcinoma (HCC) showed a correlation between incidence and geographical-relevant risk factors. This study aims to compare the distributions of cancer stem cells (CSC) in two distant populations in Asia and Europe. We analyzed 52 and 43 selected HCC patients undergoing hepatectomy in Ho Chi Minh City (Vietnam) and Trieste (Italy). Each patient sample consisted of HCC, peri-HCC, and non-tumoral (distal) tissue. Demographic data were recorded together with clinical findings. The protocol for the collection of tissue samples and RNA was standardized in both laboratories and gene expression analysis was performed in a single laboratory with identical PCR conditions. Baseline data showed comparable laboratory findings between the two cohorts. mRNA distribution showed a comparable pattern of all CSC markers analyzed with the expression of CD90 progressively increasing from distal and peri-HCC to be highest in HCC (p < 0.001), confirmed by immunofluorescence data. CD90 mRNA distribution was related to HBV-related HCC and a tumor diameter less than 5 cm. Patients with high tumoral CD90 mRNA had a shorter time (p < 0.05) to tumor recurrence compared to patients with lower CD90. This comparative study showed that CD90 mRNA expressions are comparable between Eastern and Western HCC cases. |
| Databáze: | OpenAIRE |
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