Multilayer Polyelectrolyte Films Functionalized by Insertion of Defensin: a New Approach to Protection of Implants from Bacterial Colonization
| Autor: | Catherine Picart, Joelle Ogier, Christophe Egles, Corinne Taddei, Pierre Schaaf, Youssef Haikel, Jean-Claude Voegel, Olivier Etienne, Jean-Luc Dimarcq |
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| Rok vydání: | 2004 |
| Předmět: |
Prosthesis-Related Infections
Polymers Nanotechnology medicine.disease_cause Bacterial Adhesion Defensins Electrolytes Anti-Infective Agents Polylactic Acid-Polyglycolic Acid Copolymer Escherichia coli medicine Pharmacology (medical) Lactic Acid Mechanisms of Action: Physiological Effects Defensin Pharmacology Microscopy Confocal biology Chemistry Biomaterial Membranes Artificial Prostheses and Implants Adhesion Quartz crystal microbalance biology.organism_classification Antimicrobial Polyelectrolyte Micrococcus luteus Infectious Diseases Microscopy Electron Scanning Biophysics Adsorption Polyglycolic Acid |
| Zdroj: | Antimicrobial Agents and Chemotherapy. 48:3662-3669 |
| ISSN: | 1098-6596 0066-4804 |
| DOI: | 10.1128/aac.48.10.3662-3669.2004 |
| Popis: | Infection of implanted materials by bacteria constitutes one of the most serious complications following prosthetic surgery. In the present study, we developed a new strategy based on the insertion of an antimicrobial peptide (defensin from Anopheles gambiae mosquitoes) into polyelectrolyte multilayer films built by the alternate deposition of polyanions and polycations. Quartz crystal microbalance and streaming potential measurements were used to follow step by step the construction of the multilayer films and embedding of the defensin within the films. Antimicrobial assays were performed with two strains: Micrococcus luteus (a gram-positive bacterium) and Escherichia coli D22 (a gram-negative bacterium). The inhibition of E. coli D22 growth at the surface of defensin-functionalized films was found to be 98% when 10 antimicrobial peptide layers were inserted in the film architecture. Noticeably, the biofunctionalization could be achieved only when positively charged poly( l -lysine) was the outermost layer of the film. On the basis of the results of bacterial adhesion experiments observed by confocal or electron microscopy, these observations could result from the close interaction of the bacteria with the positively charged ends of the films, which allows defensin to interact with the bacterial membrane structure. These results open new possibilities for the use of such easily built and functionalized architectures onto any type of implantable biomaterial. The modified surfaces are active against microbial infection and represent a novel means of local host protection. |
| Databáze: | OpenAIRE |
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