Responses of the insulin signaling pathways in the brown adipose tissue of rats following cold exposure
| Autor: | Xiaofei Wang, Richard L. Wahl |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Cell signaling
medicine.medical_treatment Glucose Transport Proteins Facilitative lcsh:Medicine Signal transduction Phospholipid signaling cascade Biochemistry 0302 clinical medicine Endocrinology Adipose Tissue Brown Brown adipose tissue Molecular Cell Biology Medicine and Health Sciences Glucose homeostasis Insulin Membrane Receptor Signaling AKT signaling cascade lcsh:Science 0303 health sciences Multidisciplinary Signaling cascades Hormone Receptor Signaling Cold Temperature medicine.anatomical_structure Carbohydrate Metabolism Female Research Article medicine.medical_specialty Cell biology MAPK signaling cascades Blotting Western Biology 03 medical and health sciences Internal medicine medicine Animals RNA Messenger Protein kinase B PI3K/AKT/mTOR pathway 030304 developmental biology Biology and life sciences lcsh:R IRS2 Receptor Insulin IRS1 Insulin receptor Metabolism Gene Expression Regulation Rats Inbred Lew Metabolic Disorders biology.protein lcsh:Q 030217 neurology & neurosurgery |
| Zdroj: | PLoS ONE PLoS ONE, Vol 9, Iss 6, p e99772 (2014) |
| ISSN: | 1932-6203 |
| Popis: | UNLABELLED The insulin signaling pathway is critical for the control of blood glucose levels. Brown adipose tissue (BAT) has also been implicated as important in glucose homeostasis. The effect of short-term cold exposure on this pathway in BAT has not been explored. We evaluated the effect of 4 hours of cold exposure on the insulin pathway in the BAT of rats. Whole genomic microarray chips were used to examine the transcripts of the pathway in BAT of rats exposed to 4°C and 22°C for 4 hours. The 4 most significantly altered pathways following 4 hours of cold exposure were the insulin signaling pathway, protein kinase A, PI3K/AKT and ERK/MAPK signaling. The insulin signaling pathway was the most affected. In the documented 142 genes of the insulin pathway, 42 transcripts (29.6%) responded significantly to this cold exposure with the least false discovery rate (Benjamini-Hochberg Multiple Testing: -log10 (p-value) = 7.18). Twenty-seven genes (64%) were up-regulated, including the insulin receptor (Insr), insulin substrates 1 and 2 (Irs1 and Irs2). Fifteen transcripts (36%) were down-regulated. Multiple transcripts of the primary target and secondary effector targets for the insulin signaling were also up-regulated, including those for carbohydrate metabolism. Using western blotting, we demonstrated that the cold induced higher Irs2, Irs1, and Akt-p protein levels in the BAT than in the BAT of controls maintained at room temperature, and higher Akt-p protein level in the muscle. CONCLUSION this study demonstrated that 4 hours of cold exposure stimulated the insulin signaling pathway in the BAT and muscle of overnight fasted rats. This raises the possibility that acute cold stimulation may have potential to improve glucose clearance and insulin sensitivity. |
| Databáze: | OpenAIRE |
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