Proteomic landscape of liver tissue in old male mice that are long-term treated with polysaccharides from Sargassum fusiforme
| Autor: | Cuiping You, Haibin Tong, Xu Zhang, Hongjun Chen, Man Xu, Peichao Chen, Huixi Zou, Mingjiang Wu, Ya Zhang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Proteomics Aging Antioxidant medicine.medical_treatment Polysaccharide Antioxidants 03 medical and health sciences Mice 0302 clinical medicine Polysaccharides medicine Animals Gel electrophoresis chemistry.chemical_classification Mice Inbred ICR biology Sargassum General Medicine biology.organism_classification Brown algae 030104 developmental biology Enzyme Biochemistry chemistry Liver Catalase 030220 oncology & carcinogenesis biology.protein Function (biology) Food Science |
| Zdroj: | Foodfunction. 11(4) |
| ISSN: | 2042-650X |
| Popis: | Sargassum fusiforme is a type of brown algae and well known as a longevity promoting vegetable in Northeastern Asia. The polysaccharides derived from Sargassum fusiforme (SFPs) have been suggested as an antioxidant component for anti-aging function. However, global molecular changes in vivo by SFPs have not been fully elucidated. Here, we present a proteomics study using liver tissues of aged male mice that were fed with SFPs. Of forty-nine protein spots, thirty-eight were up-regulated and eleven were down-regulated, showing significant changes in abundance by two-dimensional gel electrophoresis. These differentially expressed proteins were mainly involved in oxidation-reduction, amino acid metabolism, and energy metabolism. Forty-six proteins were integrated into a unified network, with catalase (Cat) at the center. Intriguingly, most of the proteins were speculated as mitochondrial-located proteins. Our findings suggested that SFPs modulated antioxidant enzymes to scavenge redundant free radicals, thus preventing oxidative damage. In conclusion, our study provides a proteomic view on how SFPs have beneficial effects on the aspects of antioxidant and energy metabolism during the aging process. This study facilitates the understanding of anti-aging molecular mechanisms in polysaccharides derived from Sargassum fusiforme. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|