Capillary electrophoresis of DNA restriction fragments with solutions of entangled polymers
| Autor: | Gerhard Schomburg, Maria Helen Kleemiß, Martin Gilges |
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| Rok vydání: | 1993 |
| Předmět: |
Electrophoresis
Restriction Mapping Clinical Biochemistry Polyacrylamide Buffers engineering.material Biochemistry Analytical Chemistry chemistry.chemical_compound Adsorption Capillary electrophoresis Coating Polymer chemistry Cellulose chemistry.chemical_classification integumentary system DNA Polymer Buffer solution Separation process Solutions chemistry Chemical engineering Polyvinyl Alcohol engineering Electrophoresis Polyacrylamide Gel Hydroxyethyl cellulose |
| Zdroj: | Electrophoresis. 14:515-522 |
| ISSN: | 1522-2683 0173-0835 |
| Popis: | Separation of DNA restriction fragments in dilute buffer solutions of network forming polymers such as linear polyacrylamide (PAA), hydroxyethyl cellulose (HEC) and polyvinylalcohol (PVA) in phosphate buffer were investigated. PVA in buffers already became inhomogeneous after a few separations with resultant deterioration of resolution. Hydroxylic polymers, capable of forming suitable networks in buffers, are strongly adsorbed to fused silica surfaces suppressing the electroosmotic flow. These polymers could be applied as surface modifiers in capillaries, filled with buffer media containing other polymers such as PAA: Suppression of the electroosmotic flow by adsorbed HEC at pH 7 was slightly more effective than with PVA but the former coating was less stable due to weaker binding to the fused silica surface. Good separations of restriction fragments could be achieved with solutions of PAA or HEC as separation media after surface pretreatment of fused silica capillaries by either PVA rinsing or coating according to Hjerten [41]. |
| Databáze: | OpenAIRE |
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